Anti-PIDD1 antibody (ab232773)
Key features and details
- Rabbit polyclonal to PIDD1
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PIDD1 antibody
See all PIDD1 primary antibodies -
Description
Rabbit polyclonal to PIDD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human PIDD1 aa 694-885. (Expressed in E.coli).
Database link: Q9HB75 -
Positive control
- WB: Recombinant human PIDD1 protein; HEK-293T whole cell lysate; Rat liver tissue lysate. IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232773 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 100 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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Notes |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 100 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
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Relevance
The death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD. PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli, via interaction with caspase-2 and CRADD/RAIDD. PIDD also promotes apoptosis downstream of p53 as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. PIDD has also been shown to interact with NEMO/IKBKG and RIP1 and enhance sumoylation and ubiquitination of NEMO/IKBKG, an important component for activation of the transcription factor NF-kappa-B. -
Cellular localization
Cytoplasm. Nucleus. -
Database links
- Entrez Gene: 55367 Human
- Entrez Gene: 293625 Rat
- Omim: 605247 Human
- SwissProt: Q9HB75 Human
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Alternative names
- DKFZp434D229 antibody
- Leucine rich repeats and death domain containing antibody
- Leucine-rich repeats and death domain containing antibody
see all
Images
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Formalin-fixed, paraffin-embedded human kidney tissue stained for PIDD1 using ab232773 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Anti-PIDD1 antibody (ab232773) at 1 µg/ml + Recombinant human PIDD1 protein
Developed using the ECL technique.
Predicted band size: 100 kDa -
Anti-PIDD1 antibody (ab232773) at 1 µg/ml + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Developed using the ECL technique.
Predicted band size: 100 kDa -
Anti-PIDD1 antibody (ab232773) at 1 µg/ml + Rat liver tissue lysate
Developed using the ECL technique.
Predicted band size: 100 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232773 has not yet been referenced specifically in any publications.