Overview

  • Product name

  • Description

    Rabbit polyclonal to PIDD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment (His-T7-tag) corresponding to Human PIDD1 aa 694-885. (Expressed in E.coli).
    Database link: Q9HB75

  • Positive control

    • WB: Recombinant human PIDD1 protein; HEK-293T whole cell lysate; Rat liver tissue lysate. IHC-P: Human kidney tissue.
  • General notes

     This product was previously labelled as LRDD

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 100 kDa.
IHC-P Use a concentration of 5 - 20 µg/ml.

Target

  • Relevance

    The death domain (DD) containing protein PIDD is a p53 target gene in an erythroleukemia cell line that undergoes G1 phase arrest and subsequent apoptosis after p53 expression. Independently, PIDD was also described as a DD-containing protein with unknown function. The N-terminal region of PIDD contains seven leucine-rich repeats (LRRs), a protein interaction motif found in various proteins with diverse functions, followed by two ZU-5 domains and a C-terminal DD. PIDD forms a complex with caspase-2 and the adaptor protein RAIDD. Increased PIDD expression results in spontaneous activation of caspase-2 and sensitization to apoptosis by genotoxic stimuli, via interaction with caspase-2 and CRADD/RAIDD. PIDD also promotes apoptosis downstream of p53 as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. PIDD has also been shown to interact with NEMO/IKBKG and RIP1 and enhance sumoylation and ubiquitination of NEMO/IKBKG, an important component for activation of the transcription factor NF-kappa-B.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Database links

  • Alternative names

    • DKFZp434D229 antibody
    • Leucine rich repeats and death domain containing antibody
    • Leucine-rich repeats and death domain containing antibody
    • MGC16925 antibody
    • p53-induced protein with a death domain antibody
    • PIDD antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for PIDD1 using ab232773 at 20 μg/ml in immunohistochemical analysis. DAB staining.

  • Anti-PIDD1 antibody (ab232773) at 1 µg/ml + Recombinant human PIDD1 protein

    Developed using the ECL technique.

    Predicted band size: 100 kDa

  • Anti-PIDD1 antibody (ab232773) at 1 µg/ml + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 100 kDa

  • Anti-PIDD1 antibody (ab232773) at 1 µg/ml + Rat liver tissue lysate

    Developed using the ECL technique.

    Predicted band size: 100 kDa

References

ab232773 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab232773.
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