Anti-PIF1 antibody (ab192369)
Key features and details
- Rabbit polyclonal to PIF1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-PIF1 antibody -
Description
Rabbit polyclonal to PIF1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human PIF1.
Database link: Q9H611 -
Positive control
- Hela, Raw264.7 and PC12 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab192369 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 70 kDa. |
Target
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Function
DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity. -
Tissue specificity
Weak ubiquitous expression. -
Sequence similarities
Belongs to the helicase family. PIF1 subfamily. -
Cellular localization
Mitochondrion and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 80119 Human
- Entrez Gene: 208084 Mouse
- Entrez Gene: 367645 Rat
- Omim: 610953 Human
- SwissProt: Q9H611 Human
- SwissProt: Q80SX8 Mouse
- SwissProt: Q1HG60 Rat
- Unigene: 112160 Human
see all -
Alternative names
- ATP dependent DNA helicase PIF1 antibody
- ATP-dependent DNA helicase PIF1 antibody
- C15orf20 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab192369 has been referenced in 1 publication.
- Wang J et al. PIF1 Affects the Proliferation and Apoptosis of Cervical Cancer Cells by Influencing TERT. Cancer Manag Res 12:7827-7835 (2020). PubMed: 32943924