Key features and details
- Sensitivity: 0.5 ng/ml
- Range: 0.62 ng/ml - 150 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Product namePig IFN gamma ELISA Kit
See all Interferon gamma kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 0.5 ng/ml
Range0.62 ng/ml - 150 ng/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 86.23 77% - 94% Serum 106.7 95% - 114% Plasma 88.19 79% - 97%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Pig
Abcam’s IFN gamma Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Pig IFN gamma in serum, plasma (using EDTA and Citrate as an anticoagulant, heparin is not recommended) and cell culture supernatants.
This assay employs an antibody specific for Pig IFN gamma coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN gamma present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Pig IFN gamma antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN gamma bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 360X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Pig IFN gamma 2 vials IFN gamma Microplate (12 x 8 wells) 1 unit Recombinant Pig IFN gamma Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionProduced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Tissue specificityReleased primarily from activated T lymphocytes.
Involvement in diseaseIn Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
Sequence similaritiesBelongs to the type II (or gamma) interferon family.
modificationsProteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
- Information by UniProt
- IF 1
ab113353 has been referenced in 3 publications.
- Huang G et al. Increased Neutralizing Antibody Production and Interferon-? Secretion in Response to Porcine Reproductive and Respiratory Syndrome Virus Immunization in Genetically Modified Pigs. Front Immunol 8:1110 (2017). PubMed: 28955336
- Pickering BS et al. Protection against henipaviruses in swine requires both, cell-mediated and humoral immune response. Vaccine 34:4777-86 (2016). PubMed: 27544586
- Ju XH et al. Heat stress upregulation of Toll-like receptors 2/4 and acute inflammatory cytokines in peripheral blood mononuclear cell (PBMC) of Bama miniature pigs: an in vivo and in vitro study. Animal 8:1462-8 (2014). PubMed: 24912383