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Immunology Immunoglobulins Heavy Chain IgM
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Pig IgM ELISA Kit (ab190537)

  • Datasheet
  • SDS
  • Protocol Booklet
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Standard Curve

    Key features and details

    • Sensitivity: 5.072 ng/ml
    • Range: 12.5 ng/ml - 400 ng/ml
    • Sample type: Plasma, Serum
    • Detection method: Colorimetric
    • Assay type: Sandwich (quantitative)
    • Reacts with: Pig

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    Product image
    Pig IgA ELISA Kit (ab190536)

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    Overview

    • Product name

      Pig IgM ELISA Kit
      See all IgM kits
    • Detection method

      Colorimetric
    • Precision

      Intra-assay
      Sample n Mean SD CV%
      Overall < 10%
      Inter-assay
      Sample n Mean SD CV%
      Overall < 10%
    • Sample type

      Serum, Plasma
    • Assay type

      Sandwich (quantitative)
    • Sensitivity

      5.072 ng/ml
    • Range

      12.5 ng/ml - 400 ng/ml
    • Recovery

      Sample specific recovery
      Sample type Average % Range
      Serum > 85 % - %
    • Assay duration

      Multiple steps standard assay
    • Species reactivity

      Reacts with: Pig
    • Product overview

      Abcam’s IgM Pig ELISA kit (ab190537) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgM in pig serum and plasma.

      In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

    • Platform

      Microplate

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Wash Buffer Concentrate 1 x 50ml
      5X Diluent Concentrate 1 x 50ml
      Chromogen Substrate Solution 1 x 12ml
      IgM Pig Antibody coated microwells 1 unit
      IgM Pig Calibrator 1 vial
      IgM Pig HRP Conjugate 1 vial
      Stop Solution 1 x 12ml
    • Research areas

      • Immunology
      • Immunoglobulins
      • Heavy Chain
      • IgM
      • Kits/ Lysates/ Other
      • Kits
      • ELISA Kits
      • ELISA Kits
      • Immunoglobulins ELISA kits
    • Relevance

      IgM normally constitutes about 10% of serum immunoglobulins. IgM antibody is prominent in early immune responses to most antigens and predominates in certain antibody responses such as 'natural' blood group antibodies. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. The gene for the mu constant region contains four domains separated by short intervening sequences. Class specific anti immunoglobulin antibodies are useful for: The characterization of malignant B cell proliferations. All but acute lymphocytic leukemias share either surface or intra cytoplasmic Ig with an isotypic restriction, which suggest the monoclonal nature of the cell population. Most of the chronic lymphocytic leukemias, non Hodgkin lymphomas and Burkitt's lymphoma bear surface IgM, whereas plasmocytes from Waldenström's disease bear intracytoplasmic IgM. The other isotypes are less frequently found. On the other hand multiple myelomas are usually of the IgG or IgA type. Characterization of plasma cells in inflammatory conditions: Plasma cell typing can be of use for the classification of intestinal inflammatory conditions such as inflammatory bowel disease and allergic conditions. In the latter a specific increase in the number of IgE plasma cells can be demonstrated.
    • Alternative names

      • AGM1
      • Constant region of heavy chain of IgM
      • DKFZp686I15196
      • DKFZp686I15212
      • FLJ00385
      • Hepatitis B virus receptor binding protein
      • Ig mu chain C region
      • Immunoglobulin heavy constant mu
      • Immunoglobulin M
      • Immunoglobulin mu chain
      • MGC104996
      • MGC52291
      • MU
      • VH
      see all

    Associated products

      Images

      • Standard Curve
        Standard Curve

        Representative standard curve using ab190537 IgM Pig ELISA Kit.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab190537? Please let us know so that we can cite the reference in this datasheet.

      ab190537 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Sow Colostrum and Milk Analysis

      Excellent Excellent 5/5 (Ease of Use)
      Abreviews
      Abreviews
      abreview image
      This kit was used to analyse the IgM content of sows' colostrum/milk at 0, 12 and 24 hours post-partum. Optimal dilutions for the samples were 1 in 100,000 for the 0 hour samples and 1 in 80,000 for the 12 and 24 hour samples. The kit is rapid to use and results are known within 1.5 hours including manual plate washing. The green diluent is particularly useful when plate washing as it is easier to see that all the liquid has been removed before the first wash. t should be noted that plate sealers are not included.
      Overall the kit is fast and easy to use.

      Mr. Oliver Ashton

      Verified customer

      Submitted Dec 18 2020

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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