• Product name
    Pig IL-1 beta ELISA Kit
    See all IL1 beta kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 6 pg/ml
  • Range
    8.23 pg/ml - 6000 pg/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 96.91 89% - 104%
    Serum 97.32 88% - 105%
    Plasma 87.75 80% - 92%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Pig
  • Product overview

    Abcam's IL1 beta Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of pig IL1 beta in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for IL1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent C 1 x 30ml
    Biotinylated anti-Pig IL-1 beta 2 vials
    IL1 beta Microplate (12 x 8 wells) 1 unit
    Recombinant Pig IL-1 beta Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Function
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • Sequence similarities
    Belongs to the IL-1 family.
  • Domain
    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • Cellular localization
    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • Alternative names
    • Catabolin
    • H1
    • Hematopoietin 1
    • IFN beta inducing factor
    • IL 1
    • IL 1 beta
    • IL 1B
    • IL-1 beta
    • IL1
    • IL1 BETA
    • IL1B
    • IL1B_HUMAN
    • IL1F2
    • Interleukin 1 beta
    • Interleukin 1 beta precursor
    • Interleukin-1 beta
    • LAF
    • OAF
    • Osteoclast activating factor
    • Preinterleukin beta
    • Pro interleukin 1 beta
    see all

Associated products


Our Abpromise guarantee covers the use of ab100754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab100754



ab100754 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Pig Serum (cell extracts)
Type of Kit Used
cell extracts
Did you use the Abcam Kit protocol?
Amount of Sample
100 µg
Positive control
standard provided by kit

Dr. Carolina Velez

Verified customer

Submitted Feb 22 2016

Abreview for IL1 beta Pig ELISA Kit

Excellent Excellent 5/5 (Ease of Use)

Sample: Pig Serum (Blood)
Specification: Blood
Amount of Sample: 100 µg
Type of Kit Used: ELISA
Positive control: Standards IL1 Beta
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Serum samples were analyzed undiluted, all samples were within the detection range of the kit, and the calibration curve was well adjusted (R2: 0.9992).

Abcam user community

Verified customer

Submitted Mar 26 2013

Thank you for contacting us.

I have received following reply from my colleague in laboratory;

I’m not exactly sure what the customer means by “one recovery at a high concentration” as the Spiking and Recovery QC data...

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Thank you for contacting us.

This kit is developed by one of our collaborator. Our collaborator product as this one has passed strict quality checks we have at Abcam which is why we are selling this kit. The product has been fully tested with ...

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