For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
The data sheet just gives one recovery at a high concentration, probably a spike in? I was looking to see some endogenous levels. There is also no mention of lipid interference. If the kits are extensively tested would you have more data than the data sheet
Asked on Aug 24 2012
Thank you for contacting us.
I have received following reply from my colleague in laboratory;
I’m not exactly sure what the customer means by “one recovery at a high concentration” as the Spiking and Recovery QC data only gives percentages not concentrations.
It sounds like though the customer is simply asking about the normal (serum?) levels of IL-1 beta, i.e., non-spiked samples. If so, when we tested normal pig serum with this kit we measured levels ranging from below this sensitivity limit (6 pg/ml) to 100 pg/ml. Accordingly, this is why we recommend starting with only a 2-fold dilution for serum and plasma samples.
In regards to lipid interference, this was not specifically tested. However, in general samples with high lipid levels can exhibit matrix effects (i.e. interference by affecting immunoreactivity of the target molecule, possibly by sequestering binding sites). For this reason, we rarely recommend running serum or plasma samples undiluted, even for low abundant cytokines such as IL-1 beta.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
Answered on Aug 24 2012