Overview

  • Product name

    Pig IL-17 ELISA Kit
    See all IL-17 kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    14 pg/ml
  • Range

    14 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum = 89.69 82% - 98%
    Plasma = 85.28 70% - 108%
    Cell culture media = 97.43 87% - 116%

  • Assay duration

    One step assay
  • Species reactivity

    Reacts with: Horse, Cow, Pig
  • Product overview

    Abcam’s IL-17 Pig ELISA Kit (ab193732) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-17 in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for porcine IL-17 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures IL-17 present in the samples. The wells are washed and biotinylated anti-porcine IL-17 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of IL-17 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    900x HRP-Streptavidin Concentrate 1 x 200µl
    Biotinylated porcine IL-17A detection antibody (lyophilized) 2 vials
    porcine IL-17A standards (lyophilized) 2 vials
    Pre-coated porcine IL-17A Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.
  • Tissue specificity

    Restricted to activated memory T-cells.
  • Sequence similarities

    Belongs to the IL-17 family.
  • Post-translational
    modifications

    Found both in glycosylated and nonglycosylated forms.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • CTLA 8
    • CTLA-8
    • CTLA8
    • Cytotoxic T lymphocyte associated antigen 8
    • Cytotoxic T lymphocyte associated protein 8
    • Cytotoxic T lymphocyte associated serine esterase 8
    • Cytotoxic T-lymphocyte-associated antigen 8
    • IL 17
    • IL 17A
    • IL-17
    • IL-17A
    • IL17
    • IL17_HUMAN
    • Il17a
    • Interleukin 17A
    • Interleukin-17A
    • Interleukin17
    • Interleukin17A
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:

See 1 Publication for this product

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