Overview

  • Product name

    Pig IL-6 ELISA Kit
    See all IL-6 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 45 pg/ml
  • Range

    40.96 pg/ml - 10000 pg/ml
  • Recovery

    89 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 108.3 95% - 117%
    Serum 93.47 85% - 102%
    Plasma 85.73 67% - 99%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Pig
  • Product overview

    Abcam's IL-6 (Interleukin-6) Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of porcine IL-6 in serum, plasma and cell culture supernatants. 


    This assay employs an antibody specific for pig IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    320X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated anti-Pig IL-6 2 vials
    IL-6 Microplate (12 x 8 wells) 1 unit
    Recombinant Pig IL-6 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in disease

    Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similarities

    Belongs to the IL-6 superfamily.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Interleukin BSF 2
    • B cell differentiation factor
    • B cell stimulatory factor 2
    • B-cell stimulatory factor 2
    • BSF 2
    • BSF-2
    • BSF2
    • CDF
    • CTL differentiation factor
    • Cytotoxic T cell differentiation factor
    • Hepatocyte stimulating factor
    • Hepatocyte stimulatory factor
    • HGF
    • HSF
    • Hybridoma growth factor
    • Hybridoma growth factor Interferon beta-2
    • Hybridoma plasmacytoma growth factor
    • IFN-beta-2
    • IFNB2
    • IL 6
    • IL-6
    • IL6
    • IL6_HUMAN
    • Interferon beta 2
    • Interferon beta-2
    • Interleukin 6
    • Interleukin 6 (interferon beta 2)
    • Interleukin BSF 2
    • Interleukin-6
    see all

Applications

Our Abpromise guarantee covers the use of ab100755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab100755

Protocols

References

This product has been referenced in:

  • Zhang ZB  et al. Normothermic Machine Perfusion Protects Against Liver Ischemia-Reperfusion Injury During Reduced-Size Liver Transplantation in Pigs. Ann Transplant 24:9-17 (2019). Read more (PubMed: 30607000) »
  • Manzke NE  et al. Efficacy of energy supplementation on growth performance and immune response of suckling pigs. J Anim Sci 96:4723-4730 (2018). Read more (PubMed: 30476156) »
See all 10 Publications for this product

Customer reviews and Q&As

1-6 of 6 Q&A

Question
Answer

As an example, Il-6 in the tested pig plasma is ˜2ng/ml.

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Answer

I can confirm that the requested kits (ab100755, ab100756 and ab113352) are compatible with citrate as anticoagulant. The sensitivity will not change due to different anticoagulants.

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Answer

DISCOUNT CODE: ******
Expiration date: DD MM YYYY

I am very pleased to hear you would like to accept our offer and test ab100755 in Pig tissue samples. This code will give you 1 free kit or value off of order before the expiration date. To redeem this offer, please submit an Abreview forPig tissue samplesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

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Question
Answer

Gracias por contactarnos.

Me comentan mis compañeros que aunque este kit nunca se ha testado con tejidos, dada su versatilidad con distintos tipos de muestras, es muy posible que también funcione con homogenado de cerebro.

En caso de usar el kit en este tipo de muestra, os aconsejo diluir las muestras al menos 5 veces con Assay Diluent (Item E) para minimizar los efectos de los detergentes en el buffer de lisis. Es muy posible que requieran mayor dilución, pero tendrá que determinarse empíricamente durante el experimento.

Para el lisado original lo ideal sería comenzar por 1 mg/ml de proteína, o si es posible mas, para alcanzar, una vez diluido, 50-500ug /ml. De nuevo, este rango debe ser tomado como punto de partida y tendrá que ser optimizado empíricamente.

Para preparar el buffer de lisis, quizás os puedan ayudar los siguientes consejos:

En general un buffer de lisis debe cumplir los siguientes requisitos:

a) Bajo contenido en sal (700 mM o menos).

b) No contener azida sódica.

c) No contener >0.1% SDS.

d) No contener > 10mM de agentes reductores (BME o dithiothreitol).

Esto incluiría cualquier buffer usado par a IP, incluido RIPA buffer.

En Abcam ofrecemos un servicio de evaluación de anticuerpos y demás productos, llamado Abreview, por el cual os ofrecemos un kit gratuito para una futura compra si adquirís el producto y lo testáis en una aplicación, especie o tipo de muestra no testada hasta ahora, y publicáis los resultados en nuestra website, en forma de Abreview, independientemente de que éstos sean positivos o negativos.

Para participar en el sistema Abreview:

1. Contesta a este mail indicando tu interés en participar en el sistema Abreview probando el kit ab100755 en muestras de tejido porcino. En ese momento te enviaremos un código de descuento. Dicho código debe emitirse antes de la compra de ab100755.

2. Realiza la compra del kit (por mail, fax, online o por teléfono).

3. Prueba el kit ab100755 en muestras de tejido.

4. A través del sistema Abreview, comparte tus resultados con nosotros, tanto si son positivos, como negativos (este proceso puede durar unos 10 minutos. Valoramos enormemente si pudierais adjuntar imágenes al Abreview!).

5. Una vez hayáis publicado la reseña, el código de descuento se activa. Simplemente solicita el pedido por teléfono, fax, o a través de la web, sin olvidar mencionar el código de descuento. El descuento puede ser canjeado por cualquier otro kit y es válido durante 4 meses una vez emitido.

No dudes en ponerte en contacto con nosotros si tienes cualquier otra consulta sobre esta oferta y estaré encantado de poder ayudarte.

Las condiciones de uso de esta oferta puede usted consultarlas en www.abcam.com/collaborationdiscount.

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Question

As I’m not familiar with these items, I tried to find the ab codes through your site.
For the items I found a relative code I have update the list.
For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
So please check the list again and advice.
https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
Porcine Noradrenalin ELISA kit 96 Tests (No product match)
Porcine Adrenaline ELISA kit 96 Tests (No product match)
https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

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Answer

I have received information regarding

ab119589 Cystatin C Human ELISA Kit
ab119590 Cystatin C Mouse ELISA Kit
ab119596 KIM1 Mouse ELISA Kit
ab119597 KIM1 Rat ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit

these has not been tested with Swine or pig samples so we are unable to confirm the species cross reactivity. It mean only ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed. Would you like to have a quote for these antibodies.

I hope this info will be helpful. Should you require more information please do not hesitate to contact me.

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Question

As I’m not familiar with these items, I tried to find the ab codes through your site.
For the items I found a relative code I have update the list.
For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
So please check the list again and advice.
https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
Porcine Noradrenalin ELISA kit 96 Tests (No product match)
Porcine Adrenaline ELISA kit 96 Tests (No product match)
https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

Read More
Answer

Thank you for contacting us.


To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the details soon.

The other kits ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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