Key features and details
- Sensitivity: 10 pg/ml
- Range: 8.19 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Pig
Product namePig IL-8 ELISA Kit
See all IL-8 kits
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 10 pg/ml
Range8.19 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 75.76 68% - 83% Serum 74.89 67% - 82% Plasma 73.97 67% - 81%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Pig
Abcam’s IL-8 Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Pig IL-8 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Pig IL-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Pig IL-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Pig IL-8 (lyophilized) 2 vials IL-8 Microplate (12 x 8 wells) 1 unit Recombinant Pig IL-8 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
FunctionIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
modificationsSeveral N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
- Information by UniProt
- Beta thromboglobulin like protein
ab113352 has not yet been referenced specifically in any publications.