Overview

  • Product name

    Pig IL-8 ELISA Kit
    See all IL-8 kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    8.19 pg/ml - 2000 pg/ml
  • Recovery

    74 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.76 68% - 83%
    Serum 74.89 67% - 82%
    Plasma 73.97 67% - 81%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Pig
  • Product overview

    Abcam’s IL-8 Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Pig IL-8 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Pig IL-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Pig IL-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Pig IL-8 (lyophilized) 2 vials
    IL-8 Microplate (12 x 8 wells) 1 unit
    Recombinant Pig IL-8 Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Function

    IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
  • Sequence similarities

    Belongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications

    Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • (Ala-IL-8)77
    • (Ser-IL-8)72
    • Beta thromboglobulin like protein
    • C X C motif chemokine 8
    • C-X-C motif chemokine 8
    • Chemokine (C X C motif) ligand 8
    • CXC chemokine ligand 8
    • CXCL 8
    • CXCL8
    • Emoctakin
    • GCP 1
    • GCP-1
    • GCP/IL-8 protein I
    • GCP/IL-8 protein II
    • GCP/IL-8 protein III
    • GCP/IL-8 protein IV
    • GCP/IL-8 protein V
    • GCP/IL-8 protein VI
    • GCP1
    • Granulocyte chemotactic protein 1
    • IL 8
    • IL-8
    • IL-8(1-77)
    • IL-8(9-77)
    • IL8
    • IL8/NAP1 form I
    • IL8/NAP1 form II
    • IL8/NAP1 form III
    • IL8/NAP1 form IV
    • IL8/NAP1 form V
    • IL8/NAP1 form VI
    • IL8_HUMAN
    • Inteleukin 8
    • Interleukin 8
    • interleukin8
    • LECT
    • LUCT
    • Lymphocyte derived neutrophil activating factor
    • Lymphocyte-derived neutrophil-activating factor
    • LYNAP
    • MDNCF
    • MDNCF-b
    • MDNCF-c
    • MONAP
    • Monocyte derived neutrophil activating peptide
    • Monocyte derived neutrophil activating protein
    • Monocyte derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil chemotactic factor
    • Monocyte-derived neutrophil-activating peptide
    • NAF
    • NAP 1
    • NAP-1
    • NAP1
    • Neutrophil activating peptide 1
    • Neutrophil activating protein 1
    • Neutrophil-activating factor
    • Neutrophil-activating protein 1
    • Protein 3 10C
    • Protein 3-10C
    • SCYB 8
    • SCYB8
    • Small inducible cytokine subfamily B member 8
    • T cell chemotactic factor
    • T-cell chemotactic factor
    • TSG 1
    • TSG1
    see all

Applications

Our Abpromise guarantee covers the use of ab113352 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

Protocols

References

ab113352 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

I can confirm that the requested kits (ab100755, ab100756 and ab113352) are compatible with citrate as anticoagulant. The sensitivity will not change due to different anticoagulants.

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Question

As I’m not familiar with these items, I tried to find the ab codes through your site.
For the items I found a relative code I have update the list.
For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
So please check the list again and advice.
https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
Porcine Noradrenalin ELISA kit 96 Tests (No product match)
Porcine Adrenaline ELISA kit 96 Tests (No product match)
https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

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Answer

I have received information regarding

ab119589 Cystatin C Human ELISA Kit
ab119590 Cystatin C Mouse ELISA Kit
ab119596 KIM1 Mouse ELISA Kit
ab119597 KIM1 Rat ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit

these has not been tested with Swine or pig samples so we are unable to confirm the species cross reactivity. It mean only ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed. Would you like to have a quote for these antibodies.

I hope this info will be helpful. Should you require more information please do not hesitate to contact me.

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Question

As I’m not familiar with these items, I tried to find the ab codes through your site.
For the items I found a relative code I have update the list.
For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
So please check the list again and advice.
https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
Porcine Noradrenalin ELISA kit 96 Tests (No product match)
Porcine Adrenaline ELISA kit 96 Tests (No product match)
https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

Read More
Answer

Thank you for contacting us.


To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the details soon.

The other kits ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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