• Product name

    Pig Lipocalin-2 ELISA Kit
    See all Lipocalin-2 / NGAL kits
  • Detection method

  • Precision

    Sample n Mean SD CV%
    U1 8 220ng/ml = 2%
    U2 8 34.2ng/ml = 4%
    P1 8 134ng/ml = 2%
    P2 8 206ng/ml = 2%
    Sample n Mean SD CV%
    U1 8 220ng/ml = 4%
    U2 8 34.2ng/ml = 5%
    P1 8 134ng/ml = 3%
    P2 8 206ng/ml = 10%
  • Sample type

    Urine, Serum, Plasma, Tissue Extracts, Tissue Culture Media
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 1 pg/ml
  • Range

    4 pg/ml - 400 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine = 99 51.5pg/ml - 350pg/ml
    Plasma = 98 44.5pg/ml - 326pg/ml

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Pig
  • Product overview

    Abcam’s Pig Lipocalin-2 ELISA Kit (ab207924) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of Neutrophil Gelatinase-Associated Lipocalin levels in urine, plasma or serum, tissue extracts or culture media samples.

    The assay is a sandwich ELISA performed in microwells coated with a mouse monoclonal antibody against pig NGAL. Bound NGAL is detected with another mouse monoclonal antibody labeled with biotin and the assay is developed with horseradish peroxidase (HRP)-conjugated streptavidin and a color-forming substrate.

  • Notes

    NGAL (neutrophil gelatinase-associated lipocalin) belongs to the lipocalin family of proteins. These are small secreted proteins characterized by their ability to bind small hydrophobic molecules in a structurally conserved pocket formed by β-pleated sheet, to bind to specific cell-surface receptors, and to form macromolecular complexes. NGAL has many synonyms: perhaps the most widely used is lipocalin 2 (LCN 2); more recently the name siderocalin has been used to express NGAL’s ability to bind bacterial   siderophores. As NGAL was first reported as oncogene 24p3 in the mouse, this name is still occasionally used to identify NGAL in other species.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)


  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    25x Wash Buffer Solution 1 x 40ml
    5x Sample Diluent Buffer 1 x 50ml
    Biotinylated Pig-NGAL Antibody 1 x 12ml
    HRP-Streptavidin Solution 1 x 12ml
    Microwell plate, 96 precoated wells 1 x 96 tests
    Pig NGAL Standard (0 pg/mL) 1 x 1ml
    Pig NGAL Standard (10 pg/mL) 1 x 1ml
    Pig NGAL Standard (100 pg/mL) 1 x 1ml
    Pig NGAL Standard (20 pg/mL) 1 x 1ml
    Pig NGAL Standard (200 pg/mL) 1 x 1ml
    Pig NGAL Standard (40 pg/mL) 1 x 1ml
    Pig NGAL Standard (400 pg/mL) 1 x 1ml
    Stop Solution 1 x 12ml
    TMB Substrate Solution 1 x 12ml
  • Research areas

  • Function

    Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
  • Tissue specificity

    Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
  • Sequence similarities

    Belongs to the calycin superfamily. Lipocalin family.
  • Cellular localization

    Secreted. Upon binding to the SLC22A17 (24p3R) receptor, it is internalized.
  • Information by UniProt
  • Alternative names

    • 24p3
    • 25 kDa alpha 2 microglobulin related subunit of MMP9
    • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
    • Alpha 2 microglobulin related protein
    • HGNC:6526
    • HNL
    • Lcn 2
    • Lcn2
    • Lipocalin-2
    • Migration stimulating factor inhibitor
    • MSFI
    • Neutrophil gelatinase associated lipocalin
    • Neutrophil gelatinase associated lipocalin precursor
    • Neutrophil gelatinase-associated lipocalin
    • NGAL
    • Oncogene 24p3
    • Oncogenic lipocalin 24p3
    • p25
    • Siderocalin
    • siderocalin LCN2
    • SV40 induced 24P3 protein
    • Uterocalin
    see all


Our Abpromise guarantee covers the use of ab207924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Abcam’s Pig Lipocalin-2 ELISA Kit (ab207924) Calibration Curve



ab207924 has not yet been referenced specifically in any publications.

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