Overview

  • Product name

  • Description

    Rabbit polyclonal to PIG3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Chimpanzee, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human PIG3.

    Read Abcam's proprietary immunogen policy (Peptide available as ab68589.)

  • Positive control

    • Recombinant Human PIG3 protein (ab95857) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Caco2, A549, WI-38, SW480, TE671, Human Lung Tissue, Human Skeletal Muscle Tissue, Human Colon Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab64798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

Target

  • Function

    May be involved in the generation of reactive oxygen species (ROS). Has low NADPH-dependent naphtoquinone reductase activity, with a preference for 1,2-naphtoquinone over 1,4-naphtoquinone. Has low NADPH-dependent diamine reductase activity (in vitro).
  • Sequence similarities

    Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • p53 induced gene 3 protein antibody
    • p53-induced gene 3 protein antibody
    • Putative quinone oxidoreductase antibody
    • QORX_HUMAN antibody
    • quinone oxidoreductase homolog antibody
    • Quinone oxidoreductase PIG3 antibody
    • TP53I3 antibody
    • Tumor protein p53 inducible protein 3 antibody
    • Tumor protein p53-inducible protein 3 antibody
    see all

Images

  • All lanes : Anti-PIG3 antibody (ab64798) at 1 µg/ml

    Lane 1 : Caco 2 whole cell lysate (ab3950)
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 3 : WI38 (Human lung fibroblast cell line) Whole Cell Lysate
    Lane 4 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 6 : Lung (Human) Tissue Lysate
    Lane 7 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 8 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa


    Exposure time: 2 minutes

References

This product has been referenced in:

  • Mungamuri SK  et al. Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters. Oncogene 0:N/A (2014). Read more (PubMed: 25023704) »
  • Kracikova M  et al. A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis. Cell Death Differ 20:576-88 (2013). Read more (PubMed: 23306555) »
See all 3 Publications for this product

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