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    piga-antibody-ab154603.pdf

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Cardiovascular Blood Erythrocytes
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Anti-PIGA antibody (ab154603)

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  • SDS
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Western blot - Anti-PIGA antibody (ab154603)

    Key features and details

    • Rabbit polyclonal to PIGA
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-PIGA antibody
      See all PIGA primary antibodies
    • Description

      Rabbit polyclonal to PIGA
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Mouse
      Predicted to work with: Human
    • Immunogen

      Recombinant fragment corresponding to a region within amino acids 270-484 of Human PIGA (UniProt ID: P37287).

    • Positive control

      • NIH 3T3 whole cell lysate
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.00
      Preservative: 0.01% Thimerosal (merthiolate)
      Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine), 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Blood
      • Erythrocytes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Positive Controls

      • NIH 3T3 whole cell lysate (ab7179)

    Applications

    Our Abpromise guarantee covers the use of ab154603 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.

    Target

    • Function

      Necessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.
    • Pathway

      Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
    • Involvement in disease

      Paroxysmal nocturnal hemoglobinuria (PNH) [MIM:300818]: A disorder characterized by hemolytic anemia with hemoglobinuria, thromboses in large vessels, and a deficiency in hematopoiesis. Clinical manifestation of red blood cell breakdown with release of hemoglobin into the urine is manifested most prominently by dark-colored urine in the morning. Note=The disease is caused by mutations affecting the gene represented in this entry.
      Multiple congenital anomalies-hypotonia-seizures syndrome 2 (MCAHS2) [MIM:300868]: An X-linked recessive developmental disorder characterized by dysmorphic features, neonatal hypotonia, myoclonic seizures, and variable congenital anomalies involving the central nervous, cardiac, and urinary systems. Most affected individuals die in infancy. Note=The disease is caused by mutations affecting the gene represented in this entry.
    • Sequence similarities

      Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
    • Cellular localization

      Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession P37287 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5277 Human
      • Entrez Gene: 18700 Mouse
      • Omim: 311770 Human
      • SwissProt: P37287 Human
      • SwissProt: Q64323 Mouse
      • Unigene: 137154 Human
      • Alternative names

        • Class A GlcNAc inositol phospholipid assembly protein antibody
        • GlcNAc PI synthesis protein antibody
        • GlcNAc-PI synthesis protein antibody
        • GPI3 antibody
        • MCAHS2 antibody
        • Phosphatidylinositol glycan anchor biosynthesis class A antibody
        • Phosphatidylinositol glycan biosynthesis class A protein antibody
        • Phosphatidylinositol N-acetylglucosaminyltransferase subunit A antibody
        • Phosphatidylinositol-glycan biosynthesis class A protein antibody
        • PIG A antibody
        • PIG-A antibody
        • Piga antibody
        • PIGA_HUMAN antibody
        see all

      Images

      • Western blot - Anti-PIGA antibody (ab154603)
        Western blot - Anti-PIGA antibody (ab154603)
        Anti-PIGA antibody (ab154603) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

        Predicted band size: 54 kDa



        10% SDS PAGE

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab154603? Please let us know so that we can cite the reference in this datasheet.

      ab154603 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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