Anti-PIGA antibody (ab154603)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-PIGA antibody
See all PIGA primary antibodies -
DescriptionRabbit polyclonal to PIGA
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse
Predicted to work with: Human
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Immunogen
Recombinant fragment corresponding to a region within amino acids 270-484 of Human PIGA (UniProt ID: P37287).
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Positive control
- NIH 3T3 whole cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
Target
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FunctionNecessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.
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PathwayGlycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
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Involvement in diseaseParoxysmal nocturnal hemoglobinuria (PNH) [MIM:300818]: A disorder characterized by hemolytic anemia with hemoglobinuria, thromboses in large vessels, and a deficiency in hematopoiesis. Clinical manifestation of red blood cell breakdown with release of hemoglobin into the urine is manifested most prominently by dark-colored urine in the morning. Note=The disease is caused by mutations affecting the gene represented in this entry.
Multiple congenital anomalies-hypotonia-seizures syndrome 2 (MCAHS2) [MIM:300868]: An X-linked recessive developmental disorder characterized by dysmorphic features, neonatal hypotonia, myoclonic seizures, and variable congenital anomalies involving the central nervous, cardiac, and urinary systems. Most affected individuals die in infancy. Note=The disease is caused by mutations affecting the gene represented in this entry. -
Sequence similaritiesBelongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
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Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
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Database links
- Entrez Gene: 5277 Human
- Entrez Gene: 18700 Mouse
- Omim: 311770 Human
- SwissProt: P37287 Human
- SwissProt: Q64323 Mouse
- Unigene: 137154 Human
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Alternative names
- Class A GlcNAc inositol phospholipid assembly protein antibody
- GlcNAc PI synthesis protein antibody
- GlcNAc-PI synthesis protein antibody
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Images
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Western blot - Anti-PIGA antibody (ab154603)Anti-PIGA antibody (ab154603) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE
Protocols
References
ab154603 has not yet been referenced specifically in any publications.
