Key features and details
- Rabbit polyclonal to PIGA
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PIGA antibody
See all PIGA primary antibodies
DescriptionRabbit polyclonal to PIGA
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Dog, Chimpanzee
Synthetic peptide corresponding to Human PIGA aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Human Whole Cell Lysates: TE 671, A498, HeLa - Hydroxyurea Treated (48hr, 2µM), Jurkat - Staurosporine Treated (24hr, 500nM), Y79, HeLa - Staurosporine Treated (24hr, 500nM), HeLa - Hydroxyurea Treated (48hr, 1µM)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69768 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
FunctionNecessary for the synthesis of N-acetylglucosaminyl-phosphatidylinositol, the very early intermediate in GPI-anchor biosynthesis.
PathwayGlycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Involvement in diseaseParoxysmal nocturnal hemoglobinuria (PNH) [MIM:300818]: A disorder characterized by hemolytic anemia with hemoglobinuria, thromboses in large vessels, and a deficiency in hematopoiesis. Clinical manifestation of red blood cell breakdown with release of hemoglobin into the urine is manifested most prominently by dark-colored urine in the morning. Note=The disease is caused by mutations affecting the gene represented in this entry.
Multiple congenital anomalies-hypotonia-seizures syndrome 2 (MCAHS2) [MIM:300868]: An X-linked recessive developmental disorder characterized by dysmorphic features, neonatal hypotonia, myoclonic seizures, and variable congenital anomalies involving the central nervous, cardiac, and urinary systems. Most affected individuals die in infancy. Note=The disease is caused by mutations affecting the gene represented in this entry.
Sequence similaritiesBelongs to the glycosyltransferase group 1 family. Glycosyltransferase 4 subfamily.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- Class A GlcNAc inositol phospholipid assembly protein antibody
- GlcNAc PI synthesis protein antibody
- GlcNAc-PI synthesis protein antibody
All lanes : Anti-PIGA antibody (ab69768) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 3 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 2µM)
Lane 4 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 5 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 6 : HeLa Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 7 : HeLa Whole Cell Lysate - Hydroxyurea Treated (48hr, 1µM)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab69768 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69768, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, MCF-7 cells at 5µg/ml.
ab69768 has not yet been referenced specifically in any publications.