Product nameAnti-PIGF antibody
See all PiGF primary antibodies
DescriptionRabbit polyclonal to PIGF
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Sheep, Horse, Cow, Cat, Dog, Pig
- Jurkat cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/12500.
FunctionInvolved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.
PathwayGlycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Sequence similaritiesBelongs to the PIGF family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- GPI11 homolog antibody
- MGC32646 antibody
- MGC33136 antibody
Anti-PIGF antibody (ab74778) at 1 µg/ml (in 5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa
Additional bands at: 36 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
This product has been referenced in:
- Yin Z et al. Macrophage-derived exosomal microRNA-501-3p promotes progression of pancreatic ductal adenocarcinoma through the TGFBR3-mediated TGF-ß signaling pathway. J Exp Clin Cancer Res 38:310 (2019). Read more (PubMed: 31307515) »