Key features and details
- Rabbit polyclonal to PiGF - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PiGF antibody - C-terminal
DescriptionRabbit polyclonal to PiGF - C-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human PiGF (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q07326
- IHC-P: Human kidney tissue. WB: HEK-293T, RAW 264.7 and H9c2 whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 25 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionInvolved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.
PathwayGlycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Sequence similaritiesBelongs to the PIGF family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- GPI11 homolog antibody
- MGC32646 antibody
- MGC33136 antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for PiGF using ab230516 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-PiGF antibody - C-terminal (ab230516) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : H9c2 (rat heart myoblast cell line) whole cell lysate
Predicted band size: 25 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230516 has been referenced in 1 publication.
- Zhao X & Wang X Candesartan targeting of angiotensin II type 1 receptor demonstrates benefits for hypertension in pregnancy via the NF-?B signaling pathway. Mol Med Rep 18:705-714 (2018). PubMed: 29845261