Overview

  • Product name

    Anti-PiGF antibody - C-terminal
    See all PiGF primary antibodies
  • Description

    Rabbit polyclonal to PiGF - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human PiGF (C terminal) conjugated to keyhole limpet haemocyanin.
    Database link: Q07326

  • Positive control

    • IHC-P: Human kidney tissue. WB: HEK-293T, RAW 264.7 and H9c2 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 30% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI.
  • Pathway

    Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • Sequence similarities

    Belongs to the PIGF family.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GPI11 homolog antibody
    • MGC32646 antibody
    • MGC33136 antibody
    • PHOSPHATIDYLINOSITOL GLYCAN antibody
    • Phosphatidylinositol glycan biosynthesis class F protein antibody
    • Phosphatidylinositol-glycan biosynthesis class F protein antibody
    • PIG F antibody
    • PIG-F antibody
    • PIGF antibody
    • PIGF_HUMAN antibody
    • PIGF1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for PiGF using ab230516 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-PiGF antibody - C-terminal (ab230516) at 1/500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : H9c2 (rat heart myoblast cell line) whole cell lysate

    Predicted band size: 25 kDa

References

This product has been referenced in:

  • Zhao X & Wang X Candesartan targeting of angiotensin II type 1 receptor demonstrates benefits for hypertension in pregnancy via the NF-?B signaling pathway. Mol Med Rep 18:705-714 (2018). Read more (PubMed: 29845261) »
See 1 Publication for this product

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