Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab238577 in the following tested applications.
|IHC-P||1/200 - 1/500.|
RelevancePIGN is a ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. PIGN transfers ethanolamine phosphate to the first alpha-1,4-linked mannose of the glycosylphosphatidylinositol precursor of GPI-anchor.
Cellular localizationEndoplasmic reticulum membrane; Multipass membrane protein
- Phosphatidylinositol glycan, class N antibody
- GPI ethanolamine phosphate transferase 1 antibody
- MCD4 antibody
Paraffin-embedded human endometrial cancer tissue stained for PIGN using ab238577 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ab238577 has not yet been referenced specifically in any publications.