Overview

  • Product name

    Anti-Pigu antibody [EPR16424] - C-terminal
    See all Pigu primary antibodies
  • Description

    Rabbit monoclonal [EPR16424] to Pigu - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pigu aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9H490

  • Positive control

    • HeLa, 293, Human fetal brain, C6, RAW 264.7, PC12 and NIH 3T3 lysates; Human liver and mouse kidney tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16424
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab192255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Component of the GPI transamidase complex. May be involved in the recognition of either the GPI attachment signal or the lipid portion of GPI.
  • Pathway

    Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • Sequence similarities

    Belongs to the PIGU family.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5430426F17Rik antibody
    • BC028278 antibody
    • Cdc91l1 antibody
    • Cell division cycle 91 like 1 protein antibody
    • Cell division cycle protein 91-like 1 antibody
    • GAB1 antibody
    • GPI transamidase component PIG-U antibody
    • GPI transamidase subunit antibody
    • Phosphatidylinositol glycan anchor biosynthesis class U antibody
    • Phosphatidylinositol glycan anchor biosynthesis class U protein antibody
    • Pigu antibody
    • PIGU_HUMAN antibody
    • Protein CDC91 like 1 antibody
    • Protein CDC91-like 1 antibody
    see all

Images

  • Anti-Pigu antibody [EPR16424] - C-terminal (ab192255) at 1/10000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa

  • Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Pigu with ab192255 at 1/1000 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

  • All lanes : Anti-Pigu antibody [EPR16424] - C-terminal (ab192255) at 1/1000 dilution

    Lane 1 : HT-1376 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa

  • All lanes : Anti-Pigu antibody [EPR16424] - C-terminal (ab192255) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : RAW 264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 50 kDa

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Pigu with ab192255 at 1/1000 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.

References

This product has been referenced in:

  • Cao J  et al. PIGU overexpression adds value to TNM staging in the prognostic stratification of patients with hepatocellular carcinoma. Hum Pathol 83:90-99 (2019). Read more (PubMed: 30171988) »
See 1 Publication for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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