Overview

  • Product name

    Anti-PIGX antibody
  • Description

    Rabbit polyclonal to PIGX
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human PIGX aa 42-230.
    Database link: Q8TBF5

  • Positive control

    • WB: Mouse kidney tissue lysate; HEK-293T whole cell lysate. IHC-P: Human placenta and rectum tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab235338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/1000 - 1/5000. Predicted molecular weight: 29 kDa.

Target

  • Function

    Essential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM.
  • Pathway

    Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • Sequence similarities

    Belongs to the PIGX family.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • GPI mannosyltransferase subunit antibody
    • Phosphatidylinositol glycan class X antibody
    • Phosphatidylinositol-glycan biosynthesis class X protein antibody
    • PIG-X antibody
    • Pigx antibody
    • PIGX_HUMAN antibody
    see all

Images

  • All lanes : Anti-PIGX antibody (ab235338) at 1/1000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 29 kDa

  • Paraffin-embedded human placenta tissue stained for PIGX with ab235338 at 1/100 dilution in immunohistochemical analysis.

  • Paraffin-embedded human rectum tissue stained for PIGX with ab235338 at 1/100 dilution in immunohistochemical analysis.

References

ab235338 has not yet been referenced specifically in any publications.

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