Key features and details
- Rabbit polyclonal to PIGX
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PIGX antibody
DescriptionRabbit polyclonal to PIGX
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human PIGX aa 42-230.
Database link: Q8TBF5
- WB: Mouse kidney tissue lysate; HEK-293T whole cell lysate. IHC-P: Human placenta and rectum tissues.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 29 kDa.|
FunctionEssential component of glycosylphosphatidylinositol-mannosyltransferase 1 which transfers the first of the 4 mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Probably acts by stabilizing the mannosyltransferase PIGM.
PathwayGlycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Sequence similaritiesBelongs to the PIGX family.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- GPI mannosyltransferase subunit antibody
- Phosphatidylinositol glycan class X antibody
- Phosphatidylinositol-glycan biosynthesis class X protein antibody
All lanes : Anti-PIGX antibody (ab235338) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29 kDa
Paraffin-embedded human placenta tissue stained for PIGX with ab235338 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human rectum tissue stained for PIGX with ab235338 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235338 has not yet been referenced specifically in any publications.