Key features and details
- Rabbit polyclonal to PIH1D1
- Suitable for: IHC-P, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PIH1D1 antibody
DescriptionRabbit polyclonal to PIH1D1
Tested applicationsSuitable for: IHC-P, ICC/IF, IPmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human PIH1D1 aa 1-290.
MANPKLLGMGLSEAEAIGADSARFEELLLQASKELQQAQTTRPESTQIQP QPGFCIKTNSSEGKVFINICHSPSIPPPADVTEEELLQMLEEDQAGFRIP MSLGEPHAELDAKGQGCTAYDVAVNSDFYRRMQNSDFLRELVITIAREGL EDKYNLQLNPEWRMMKNRPFMGSISQQNIRSEQRPRIQELGDLYTPAPGR AESGPEKPHLNLWLEAPDLLLAEVDLPKLDGALGLSLEIGENRLVMGGPQ QLYHLDAYIPLQINSHESKAAFHRKRKQLMVAMPLLPVPS
Database link: Q9NWS0-1
- IHC-P: Human placenta tissue. ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab238862 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/500.|
|IP||1/200 - 1/2000.|
Sequence similaritiesBelongs to the PIH1 family.
- Information by UniProt
- NOP17 antibody
- Nucleolar protein 17 homolog antibody
- PIH1 domain containing 1 antibody
Paraffin-embedded human placenta tissue stained for PIH1D1 using ab238862 at 1/100 dilution in immunohistochemical analysis.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for PIH1D1 (green) using ab238862 at 1/200 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counterstain is DAPI.
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.
PIH1D1 was immunoprecipitated from 0.5 mg HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab238862 at 1/200 dilution.
Lane 1: Control rabbit IgG IP (1 μg) in HeLa whole cell lysate.
Lane 2: ab238862 IP in HeLa whole cell lysate.
Lane 3: HeLa whole cell lysate 10 μg (Input).
For Western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238862 has been referenced in 1 publication.
- Morwitzer MJ et al. Identification of RUVBL1 and RUVBL2 as Novel Cellular Interactors of the Ebola Virus Nucleoprotein. Viruses 11:N/A (2019). PubMed: 31018511