FormLyophilised:Reconstitute the antibody with 100 uL sterile PBS and the final concentration is 500 ug/mL. Reconstituted antibody can also be aliquoted and stored frozen at < -20 °C for at least for six months without detectable loss of activity
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe IgG fraction of immunized serum was purified by antigen affinity chromatography and lyophilized from a 0.2 µm filtered solution in phosphate-buffered saline (PBS).
Our Abpromise guarantee covers the use of ab214924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 185 kDa.|
RelevanceThe protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner.
Cellular localizationMicrosome. Cell membrane. Cytoplasm, cytosol. Nucleus. Note=Found mostly in the microsome, but also in the plasma membrane and cytosol. Nuclear in testis.
- C2 PI3K antibody
- phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide antibody
- phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta antibody
ab214924 has not yet been referenced specifically in any publications.