Key features and details
- Rabbit polyclonal to PIK3C2B
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-PIK3C2B antibody
See all PIK3C2B primary antibodies
DescriptionRabbit polyclonal to PIK3C2B
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment (His-T7-tag) corresponding to Rat PIK3C2B aa 790-1025. Expressed in E.coli. N-terminal tags.
SPRYEFGSLREEDQRKLKDITQKESLYWLSDADKKQLWEKRYYCHTEVSS LPLVLASAPSWEWACLPDIYALLQQWTHMNHQDALGLLHATFPDQEVRRM AVQWIGSLSDAELLDYLPQLVQALKYECYLDSPLVRFLLKRAISDLRVTH YFFWLLKDSLKDSQFSIRYQYLLAALLCCCGKGLREEFNRQCWLVNTLAK LAQQVREATPSARQGILRAGLEEVRQFFALNGSCRL
Database link: D3ZVF3
- IHC-P: Rat lymph node tissue. WB: Rat serum; Recombinant rat PIK3C2B protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231122 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 183 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
RelevanceThe protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is sensitive to low nanomolar levels of the inhibitor wortmanin. The C2 domain of this protein was shown to bind phospholipids but not Ca2+, which suggests that this enzyme may function in a calcium-independent manner.
Cellular localizationMicrosome. Cell membrane. Cytoplasm, cytosol. Nucleus. Note=Found mostly in the microsome, but also in the plasma membrane and cytosol. Nuclear in testis.
- C2 PI3K antibody
- phosphatidylinositol 3-kinase C2 domain-containing beta polypeptide antibody
- phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta antibody
Formalin-fixed, paraffin-embedded rat lymph node tissue stained for PIK3C2B using ab231122 at 20 µg/ml in immunohistochemical analysis.
Anti-PIK3C2B antibody (ab231122) at 1 µg/ml + Rat serum
Predicted band size: 183 kDa
Anti-PIK3C2B antibody (ab231122) at 2 µg/ml + Recombinant rat PIK3C2B protein
Predicted band size: 183 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231122 has been referenced in 1 publication.
- Wang C et al. Novel Potential Biomarkers Associated With Epithelial to Mesenchymal Transition and Bladder Cancer Prognosis Identified by Integrated Bioinformatic Analysis. Front Oncol 10:931 (2020). PubMed: 32695668