Overview

  • Product name

  • Description

    Rabbit polyclonal to PIKE
  • Host species

    Rabbit
  • Specificity

    Recognises PIKE: also detect both PIKE-L and PIKE-A isoforms.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human PIKE (C terminal).

  • Positive control

    • Mouse brain cell lysates.
  • General notes

    Previously labelled as Centaurin gamma 1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab21271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 150, 80 kDa (predicted molecular weight: 90 kDa).

Target

  • Function

    GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
  • Tissue specificity

    Isoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.
  • Sequence similarities

    Belongs to the centaurin gamma-like family.
    Contains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
  • Domain

    G domain binds GTP and has GTPase activity.
    Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
    Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1.
  • Post-translational
    modifications

    Isoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage.
  • Cellular localization

    Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • AGAP-2 antibody
    • Agap2 antibody
    • Agap2 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
    • AGAP2_HUMAN antibody
    • ANK repeat and PH domains 2 antibody
    • Arf GAP with GTP binding protein like antibody
    • Arf GAP with GTP-binding protein-like, ANK repeat and PH domains 2 antibody
    • Arf GAP with GTP-binding protein-like, ankyrin repeat and pleckstrin homology domains 2 antibody
    • Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 antibody
    • ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
    • Centaurin gamma 1 antibody
    • centaurin gamma1 antibody
    • Centaurin-gamma-1 antibody
    • Centg1 antibody
    • cnt g1 antibody
    • Cnt-g1 antibody
    • Cnt2 antibody
    • FLJ16430 antibody
    • GGAP2 antibody
    • GTP binding and GTPase activating protein 2 antibody
    • GTP-binding and GTPase activating protein 2 antibody
    • GTP-binding and GTPase-activating protein 2 antibody
    • KIAA0167 antibody
    • mKIAA0167 antibody
    • Nuclear GTPase PIKE antibody
    • Phosphatidylinositol 3-kinase enhancer antibody
    • Phosphoinositide 3 kinase enhancer antibody
    • PI3K ENHANCER antibody
    • PIKE antibody
    see all

Images

  • Lane 1 : Anti-PIKE antibody (ab21271) at 0.5 µg/ml
    Lane 2 : Anti-PIKE antibody (ab21271) at 1 µg/ml
    Lane 3 : Anti-PIKE antibody (ab21271) at 2 µg/ml

    All lanes : Mouse brain cell lysate

    Predicted band size: 90 kDa



    Ab21271 at concentrations of 0.5 (lane A), 1 (lane B) and 2 µg/ml (lane C), staining PIKE-L and PIKE-A in mouse brain cell lysates by Western blot.  
  • ab21271 at 10µg/ml staining PIKE in mouse brain tissue by IHC

References

ab21271 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your interest.

I have conducted a search for you and found 7 antibodies against this target (PIKE, GGAP2 AGAP2, Centaurin). Here are some which have been tested in Western blot application and Immunostaining, it seems that none of them have been characterized specifically for IP yet:

ab21271: IHC-P, WB

ab105332: IHC-P, WB

ab110956: ICC, WB

ab111987: IHC-P, WB

If you need any further assistance in the future, please do not hesitate to contact me.

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