Key features and details
- Rabbit polyclonal to PIKE
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-PIKE antibody
See all PIKE primary antibodies
DescriptionRabbit polyclonal to PIKE
SpecificityRecognises PIKE: also detect both PIKE-L and PIKE-A isoforms.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to Human PIKE (C terminal).
- Mouse brain cell lysates.
Previously labelled as Centaurin gamma 1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPurified by ion exchange chromatography.
Our Abpromise guarantee covers the use of ab21271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 150, 80 kDa (predicted molecular weight: 90 kDa).|
FunctionGTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
Tissue specificityIsoform 1 is brain-specific. Isoform 2 is ubiquitously expressed, with highest levels in brain and heart.
Sequence similaritiesBelongs to the centaurin gamma-like family.
Contains 2 ANK repeats.
Contains 1 Arf-GAP domain.
Contains 1 PH domain.
DomainG domain binds GTP and has GTPase activity.
Arf-GAP domain interacts with G domain and may regulate its GTPase activity.
Although both PH domains of isoforms 1 and 2 bind phospholipids, they differently regulate subcellular location. PH domain of isoform 1 directs the protein to the nucleus, but PH domain of isoform 2 directs it to the cytosol. PH domain of isoform 2 is required for binding to AP-1.
modificationsIsoform PIKE-A is phosphorylated at Tyr-682 and Tyr-774 by FYN, preventing its apoptotic cleavage.
Cellular localizationCytoplasm and Cytoplasm. Nucleus.
- Information by UniProt
- AGAP-2 antibody
- Agap2 antibody
- Agap2 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody
Lane 1 : Anti-PIKE antibody (ab21271) at 0.5 µg/ml
Lane 2 : Anti-PIKE antibody (ab21271) at 1 µg/ml
Lane 3 : Anti-PIKE antibody (ab21271) at 2 µg/ml
All lanes : Mouse brain cell lysate
Predicted band size: 90 kDa
Ab21271 at concentrations of 0.5 (lane A), 1 (lane B) and 2
µg/ml (lane C), staining PIKE-L and PIKE-A in mouse brain cell lysates by Western blot.
ab21271 at 10µg/ml staining PIKE in mouse brain tissue by IHC
ab21271 has not yet been referenced specifically in any publications.