Key features and details
- Mouse monoclonal [ZP003] to PIM1
- Suitable for: WB, ELISA
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-PIM1 antibody [ZP003]
See all PIM1 primary antibodies
DescriptionMouse monoclonal [ZP003] to PIM1
Specificityab54503 is specific for the PIM1 protein.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Synthetic peptide derived from the internal region of the human PIM1 protein, which is 94% and 88% homologous with rat and mouse, respectively.
- WB: K562 cytoplasmic or nuclear lysate.
Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab54503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa.|
|ELISA||Use a concentration of 0.1 - 1 µg/ml.|
FunctionMay affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
Tissue specificityExpressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated on both serine/threonine and tyrosine residues.
Cellular localizationCytoplasm. Nucleus and Cell membrane.
- Information by UniProt
- Oncogene PIM 1 antibody
- Oncogene PIM1 antibody
- PIM 1 antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PIM1 knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab54503 observed at 35 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab54503 was shown to recognize PIM1 when PIM1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PIM1 knockout samples were subjected to SDS-PAGE. ab54503 and ab181602 (loading control to GAPDH) were diluted 3 μg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
Anti-PIM1 antibody [ZP003] (ab54503) at 3 µg/ml + K562 cell lysate
Observed band size: 35 kDa why is the actual band size different from the predicted?
ab54503 has been referenced in 1 publication.
- Gao X et al. PIM1 is responsible for IL-6-induced breast cancer cell EMT and stemness via c-myc activation. Breast Cancer N/A:N/A (2019). PubMed: 30989585