Key features and details
- Rabbit polyclonal to PIM1 (phospho Y309)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-PIM1 (phospho Y309) antibody
See all PIM1 primary antibodies
DescriptionRabbit polyclonal to PIM1 (phospho Y309)
Specificityab74047 detects endogenous levels of human PIM1 only when phosphorylated at tyrosine 309. ab74047 detects endogenous levels of mouse PIM1 only when phosphorylated at tyrosine 302 and rat PIM1 only when phosphorylated at tyrosine 218.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phosphopeptide derived from human PIM1 around the phosphorylation site of tyrosine 309 (H-R-YP-H-G).
- HUVEC cell extracts treated with PMA (125ng/ml, 30mins).
Storage instructionsFrozen Stock (-20C). Shelf life 12 months.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab74047 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab74047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).|
FunctionMay affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
Tissue specificityExpressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain.
modificationsAutophosphorylated on both serine/threonine and tyrosine residues.
Cellular localizationCytoplasm. Nucleus and Cell membrane.
- Information by UniProt
- Oncogene PIM 1 antibody
- Oncogene PIM1 antibody
- PIM 1 antibody
All lanes : Anti-PIM1 (phospho Y309) antibody (ab74047) at 1/500 dilution
Lane 1 : HUVEC cell extracts
treated with PMA (125ng/ml, 30mins)
Lane 2 : HUVEC cell extracts
treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
ab74047 has not yet been referenced specifically in any publications.