• Product name

    Anti-PIM1 (phospho Y309) antibody
    See all PIM1 primary antibodies
  • Description

    Rabbit polyclonal to PIM1 (phospho Y309)
  • Host species

  • Specificity

    ab74047 detects endogenous levels of human PIM1 only when phosphorylated at tyrosine 309. ab74047 detects endogenous levels of mouse PIM1 only when phosphorylated at tyrosine 302 and rat PIM1 only when phosphorylated at tyrosine 218.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human PIM1 around the phosphorylation site of tyrosine 309 (H-R-YP-H-G).

  • Positive control

    • HUVEC cell extracts treated with PMA (125ng/ml, 30mins).



Our Abpromise guarantee covers the use of ab74047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
ELISA 1/40000.


  • Function

    May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis.
  • Tissue specificity

    Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Post-translational

    Autophosphorylated on both serine/threonine and tyrosine residues.
  • Cellular localization

    Cytoplasm. Nucleus and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Oncogene PIM 1 antibody
    • Oncogene PIM1 antibody
    • PIM 1 antibody
    • pim 1 kinase 44 kDa isoform antibody
    • Pim 1 kinase antibody
    • pim 1 oncogene (proviral integration site 1) antibody
    • Pim 1 oncogene antibody
    • PIM antibody
    • PIM1 antibody
    • pim1 kinase 44 kDa isoform antibody
    • PIM1_HUMAN antibody
    • Pim2 antibody
    • PIM3 antibody
    • Proto oncogene serine/threonine protein kinase Pim 1 antibody
    • Proto-oncogene serine/threonine-protein kinase Pim-1 antibody
    • Proviral integration site 1 antibody
    • Proviral integration site 2 antibody
    see all


  • All lanes : Anti-PIM1 (phospho Y309) antibody (ab74047) at 1/500 dilution

    Lane 1 : HUVEC cell extracts
    treated with PMA (125ng/ml, 30mins)
    Lane 2 : HUVEC cell extracts
    treated with PMA (125ng/ml, 30mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa


ab74047 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


The Pim1 antibodies we have in our catalog are : ab54457 : the epitope recognized by this antibody maps to a region between aa 250-313 and is specific to Pim-1. No cross-reactivity with Pim-2 or -3 is expected. ab74047 and ab74118 can't react with human Pim2, but the homology is 100% with human Pim3. ab75776 : This antibody is not predicted to cross react with Pim2 or Pim3. Sequence analysis shows that the immunogen shares 45% and 53% identity with Pim2 and Pim3, respectively. However, it is not guaranteed without data. ab85898 : this antibody can't detect Pim2 and Pim3, it is specific to Pim1. ab117518 : the epitope recognized by this monoclonal antibody has not been mapped so I cannot provide you with blast homology scores. The Pim1 protein we have in our catalog and which can be used as a positive control is : ab60835 The prices for each antibody as well as for the protein are indicated on the product datasheet (make sure you select the right country using the drop menu at the top right corner of the Abcam website). I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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