Product nameAnti-PIM2 antibody
See all PIM2 primary antibodies
DescriptionMouse polyclonal to PIM2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Full length protein corresponding to Human PIM2 aa 1-311.
MLTKPLQGPPAPPGTPTPPPGGKDREAFEAEYRLGPLLGKGGFGTVFAGH RLTDRLQVAIKVIPRNRVLGWSPLSDSVTCPLEVALLWKVGAGGGHPGVI RLLDWFETQEGFMLVLERPLPAQDLFDYITEKGPLGEGPSRCFFGQVVAA IQHCHSRGVVHRDIKDENILIDLRRGCAKLIDFGSGALLHDEPYTDFDGT RVYSPPEWISRHQYHALPATVWSLGILLYDMVCGDIPFERDQEILEAELH FPAHVSPDCCALIRRCLAPKPSSRPSLEEILLDPWMQTPAEDVPLNPSKG GPAPLAWSLLP
Database link: NP_006866.2
- PIM2-transfected 293T cell line lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab172652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.|
FunctionPromotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
Tissue specificityHighly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- OTTHUMP00000025825 antibody
- Pim 2 oncogene antibody
- Pim 2h antibody
All lanes : Anti-PIM2 antibody (ab172652) at 1 µg/ml
Lane 1 : PIM2-transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Mouse IgG-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 34 kDa
ab172652 has not yet been referenced specifically in any publications.