Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab177237 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab177237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.|
FunctionPromotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
Tissue specificityHighly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- OTTHUMP00000025825 antibody
- Pim 2 oncogene antibody
- Pim 2h antibody
Lane 1 : Anti-PIM2 antibody (ab177237) at 0.1 µg/ml
Lane 2 : Anti-PIM2 antibody (ab177237) at 0.1 mg/ml
Lane 3 : Anti-MYC tag at 1/1000 dilution
Lanes 1 & 3 : HEK293 lysate (in RIPA buffer) overexpressing Human PIM2 with C-terminal MYC tag
Lane 2 : Mock transfected HEK193 lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 34 kDa
Primary incubation was 1 hour
ab177237 has not yet been referenced specifically in any publications.