Overview

  • Product name
  • Description
    Rabbit polyclonal to PIM2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human PIM2 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9P1W9

  • Positive control
    • Human B cell lymphoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab93667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

1/100 for 30 minutes at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Target

  • Function
    Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
  • Tissue specificity
    Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000025825 antibody
    • Pim 2 oncogene antibody
    • Pim 2h antibody
    • Pim-2h antibody
    • PIM2 antibody
    • PIM2 Oncogene antibody
    • PIM2_HUMAN antibody
    • Pim2h antibody
    • Proto Oncogene PIM2 antibody
    • Proto-oncogene Pim-2 (serine threonine kinase) antibody
    • Serine/threonine kinase PIM2 antibody
    • Serine/threonine-protein kinase Pim-2 antibody
    see all

Images

  • ab93667, at a 1/100 dilution, staining PIM2 in formalin fixed, paraffin embedded Human B cell lymphoma tissue by Immunohistochemistry.

References

ab93667 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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