Anti-PIM2 antibody (ab93667)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-PIM2 antibody
See all PIM2 primary antibodies -
DescriptionRabbit polyclonal to PIM2
-
Host speciesRabbit
-
Tested applicationsSuitable for: IHC-Pmore details
-
Species reactivityReacts with: Human
-
Immunogen
Synthetic peptide within Human PIM2 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9P1W9 -
Positive control
- Human B cell lymphoma tissue
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C.
-
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: BSA, 0.0268% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesImmunogen affinity purified immunoglobulin from rabbit serum.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab93667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. 1/100 for 30 minutes at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
Target
-
FunctionPromotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
-
Tissue specificityHighly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
-
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated. - Information by UniProt
-
Database links
- Entrez Gene: 11040 Human
- Omim: 300295 Human
- SwissProt: Q9P1W9 Human
- Unigene: 726382 Human
-
Alternative names
- OTTHUMP00000025825 antibody
- Pim 2 oncogene antibody
- Pim 2h antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM2 antibody (ab93667)ab93667, at a 1/100 dilution, staining PIM2 in formalin fixed, paraffin embedded Human B cell lymphoma tissue by Immunohistochemistry.
References
ab93667 has not yet been referenced specifically in any publications.
