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Signal Transduction Protein Phosphorylation Ser / Thr Kinases Other Kinases
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Anti-PIM2 antibody (ab93667)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM2 antibody (ab93667)

    Key features and details

    • Rabbit polyclonal to PIM2
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant human PIM2 protein (ab60836)

    View more associated products

    Overview

    • Product name

      Anti-PIM2 antibody
      See all PIM2 primary antibodies
    • Description

      Rabbit polyclonal to PIM2
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide within Human PIM2 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
      Database link: Q9P1W9

    • Positive control

      • Human B cell lymphoma tissue
    • General notes

      This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      pH: 7.60
      Preservative: 0.1% Sodium azide
      Constituents: PBS, 1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Immunogen affinity purified immunoglobulin from rabbit serum.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Phosphorylation
      • Ser / Thr Kinases
      • Other Kinases
      • Cancer
      • Oncoproteins/suppressors
      • Oncoproteins
      • Signal transducers

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human PIM2 protein (ab60836)

    Applications

    Our Abpromise guarantee covers the use of ab93667 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    1/100 for 30 minutes at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Boil tissue section in 1mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

    Target

    • Function

      Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
    • Tissue specificity

      Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated.
    • Target information above from: UniProt accession Q9P1W9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 11040 Human
      • Omim: 300295 Human
      • SwissProt: Q9P1W9 Human
      • Unigene: 726382 Human
      • Alternative names

        • OTTHUMP00000025825 antibody
        • Pim 2 oncogene antibody
        • Pim 2h antibody
        • Pim-2h antibody
        • PIM2 antibody
        • PIM2 Oncogene antibody
        • PIM2_HUMAN antibody
        • Pim2h antibody
        • Proto Oncogene PIM2 antibody
        • Proto-oncogene Pim-2 (serine threonine kinase) antibody
        • Serine/threonine kinase PIM2 antibody
        • Serine/threonine-protein kinase Pim-2 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM2 antibody (ab93667)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM2 antibody (ab93667)
        ab93667, at a 1/100 dilution, staining PIM2 in formalin fixed, paraffin embedded Human B cell lymphoma tissue by Immunohistochemistry.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (0)

      Publishing research using ab93667? Please let us know so that we can cite the reference in this datasheet.

      ab93667 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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