RecombinantRabMAb

Recombinant Anti-PIM2 antibody [EPR6987] (ab129057)

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Western blot - Anti-PIM2 antibody [EPR6987] (ab129057)
  • Immunoprecipitation - Anti-PIM2 antibody [EPR6987] (ab129057)
  • Western blot - Anti-PIM2 antibody [EPR6987] (ab129057)
  • OI-RD Scanning - Anti-PIM2 antibody [EPR6987] (ab129057)
  • Anti-PIM2 antibody [EPR6987] (ab129057)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6987] to PIM2
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PIM2 antibody [EPR6987]
    See all PIM2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6987] to PIM2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PIM2 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat, K562, Raji, HL60 and 293T cell lysates, Human heart, Mouse heart, Rat heart, Human kidney, and Mouse kidney tissue lysates; IP: K-562 cell lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IP
Human
WB
Mouse
Rat
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 34-40 kDa (predicted molecular weight: 34 kDa).
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 34-40 kDa (predicted molecular weight: 34 kDa).
IP
1/10 - 1/100.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.

    • Tissue specificity

      Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.

    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Autophosphorylated.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • OTTHUMP00000025825 antibody
      • Pim 2 oncogene antibody
      • Pim 2h antibody
      • Pim-2h antibody
      • PIM2 antibody
      • PIM2 Oncogene antibody
      • PIM2_HUMAN antibody
      • Pim2h antibody
      • Proto Oncogene PIM2 antibody
      • Proto-oncogene Pim-2 (serine threonine kinase) antibody
      • Serine/threonine kinase PIM2 antibody
      • Serine/threonine-protein kinase Pim-2 antibody
      see all

    Images

    • Western blot - Anti-PIM2 antibody [EPR6987] (ab129057)
      Western blot - Anti-PIM2 antibody [EPR6987] (ab129057)
      All lanes : Anti-PIM2 antibody [EPR6987] (ab129057) at 1/10000 dilution (Purified)

      Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
      Lane 2 : Human heart lysates
      Lane 3 : Mouse heart lysates
      Lane 4 : Rat heart lysates
      Lane 5 : Human kidney lysates
      Lane 6 : Mouse kidney lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 34 kDa
      Observed band size: 34-44 kDa why is the actual band size different from the predicted?



      The multiple bands are consistent with what has been described in PMID: 7781606 and 24659821 

    • Immunoprecipitation - Anti-PIM2 antibody [EPR6987] (ab129057)
      Immunoprecipitation - Anti-PIM2 antibody [EPR6987] (ab129057)

      ab129057 (purified) at 1:100 dilution (2µg) immunoprecipitating PIM2 in K-562 whole cell lysate.
      Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
      Lane 2 (+): ab129057 & K-562 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129057 in K-562 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-PIM2 antibody [EPR6987] (ab129057)
      Western blot - Anti-PIM2 antibody [EPR6987] (ab129057)
      All lanes : Anti-PIM2 antibody [EPR6987] (ab129057) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : HL60 cell lysate
      Lane 5 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 34 kDa

    • OI-RD Scanning - Anti-PIM2 antibody [EPR6987] (ab129057)
      OI-RD Scanning - Anti-PIM2 antibody [EPR6987] (ab129057)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-PIM2 antibody [EPR6987] (ab129057)
      Anti-PIM2 antibody [EPR6987] (ab129057)

    References (2)

    Publishing research using ab129057? Please let us know so that we can cite the reference in this datasheet.

    ab129057 has been referenced in 2 publications.

    • Su D  et al. PTIP chromatin regulator controls development and activation of B cell subsets to license humoral immunity in mice. Proc Natl Acad Sci U S A 114:E9328-E9337 (2017). Flow Cyt ; Mouse . PubMed: 29078319
    • Horiuchi D  et al. PIM1 kinase inhibition as a targeted therapy against triple-negative breast tumors with elevated MYC expression. Nat Med 22:1321-1329 (2016). PubMed: 27775705

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