Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6988] to PIM2
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-PIM2 antibody [EPR6988]
See all PIM2 primary antibodies
DescriptionRabbit monoclonal [EPR6988] to PIM2
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PIM2 aa 250-350. The exact sequence is proprietary.
- Jurkat, K562, Raji, and HL60 cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20ºC.
Dissociation constant (KD)KD = 6.10 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 34-40 kDa (predicted molecular weight: 34 kDa).|
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
FunctionPromotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
Tissue specificityHighly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- OTTHUMP00000025825 antibody
- Pim 2 oncogene antibody
- Pim 2h antibody
All lanes : Anti-PIM2 antibody [EPR6988] (ab129193) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : K562 cell lysates
Lane 3 : Raji cell lysates
Lane 4 : HL60 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 34 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab129193 has been referenced in 1 publication.
- Hiasa M et al. Pim-2 kinase is an important target of treatment for tumor progression and bone loss in myeloma. Leukemia N/A:N/A (2014). PubMed: 24787487