Overview

  • Product name

    Anti-PIM2 antibody [EPR6988]
    See all PIM2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6988] to PIM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PIM2 aa 250-350. The exact sequence is proprietary.

  • Positive control

    • Jurkat, K562, Raji, and HL60 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34-40 kDa (predicted molecular weight: 34 kDa).
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
    • Tissue specificity

      Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications

      Autophosphorylated.
    • Information by UniProt
    • Database links

    • Alternative names

      • OTTHUMP00000025825 antibody
      • Pim 2 oncogene antibody
      • Pim 2h antibody
      • Pim-2h antibody
      • PIM2 antibody
      • PIM2 Oncogene antibody
      • PIM2_HUMAN antibody
      • Pim2h antibody
      • Proto Oncogene PIM2 antibody
      • Proto-oncogene Pim-2 (serine threonine kinase) antibody
      • Serine/threonine kinase PIM2 antibody
      • Serine/threonine-protein kinase Pim-2 antibody
      see all

    Images

    • All lanes : Anti-PIM2 antibody [EPR6988] (ab129193) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : K562 cell lysates
      Lane 3 : Raji cell lysates
      Lane 4 : HL60 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 34 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Hiasa M  et al. Pim-2 kinase is an important target of treatment for tumor progression and bone loss in myeloma. Leukemia N/A:N/A (2014). Read more (PubMed: 24787487) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab129193.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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