• Product name
    Anti-PIM3 antibody - Carboxyterminal end
    See all PIM3 primary antibodies
  • Description
    Rabbit polyclonal to PIM3 - Carboxyterminal end
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human PIM3 aa 259-288 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 415116

  • Positive control
    • WB: Mouse kidney tissue lysate IHC-P: Human hepatocarcinoma tissue



Our Abpromise guarantee covers the use of ab71321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/10 - 1/50.
ELISA 1/1000.


  • Function
    Able to phosphorylate CDKN1B in vitro. Involved in cell cycle progression and suppression of apoptosis. Implicated in proliferation of human hepatoma cell lines.
  • Tissue specificity
    Widely expressed. No expression in colon, thymus, and small intestine. Expressed in human hepatoma cell lines but not in normal liver tissues. Expressed in colon cancer samples, particularly at the less advanced stages of cancer development.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kid 1 antibody
    • Kid1 antibody
    • Kinase induced by depolarization antibody
    • ONCOGENE PIM3 antibody
    • PIM 3 antibody
    • Pim 3 oncogene antibody
    • Pim-3 proto-oncogene, serine/threonine kinase antibody
    • PIM3 antibody
    • Pim3 oncogene antibody
    • PIM3_HUMAN antibody
    • Protein kinase Kid 1 antibody
    • Protein kinase Kid1 antibody
    • Serine/threonine kinase Pim 3 antibody
    • Serine/threonine kinase Pim3 antibody
    • Serine/threonine protein kinase Pim 3 antibody
    • Serine/threonine protein kinase Pim3 antibody
    • Serine/threonine-protein kinase Pim-3 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue sections labeling PIM3 - Carboxyterminal end with ab71321 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-PIM3 antibody - Carboxyterminal end (ab71321) at 1/1000 dilution + mouse kidney tissue lysate at 12.5 µg

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 36 kDa
    Observed band size: 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.


This product has been referenced in:
  • Stafman LL  et al. Targeting PIM kinase as a therapeutic strategy in human hepatoblastoma. Oncotarget 9:22665-22679 (2018). Read more (PubMed: 29854306) »
  • Selmi T  et al. ZFP36 expression impairs glioblastoma cell lines viability and invasiveness by targeting multiple signal transduction pathways. Cell Cycle 11:1977-87 (2012). WB ; Human . Read more (PubMed: 22544323) »

See all 2 Publications for this product

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