Product nameAnti-Pin1 antibody
See all Pin1 primary antibodies
DescriptionRabbit polyclonal to Pin1
Specificityab12107 detects human Pin 1 at ~20kDa. ab12107 also detects a band at ~23kDa, which is not attenuated by blocking peptide ab12397; the identity of this band is unknown.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from residues 50 - 150 of Human Pin1.
- This antibody gave a positive result with Pin1 recombinant protein
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab12107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 18.2 kDa).|
FunctionEssential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
Sequence similaritiesContains 1 PpiC domain.
Contains 1 WW domain.
DomainThe WW domain is required for the interaction with STIL and KIF20B.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- DOD antibody
- DODO, Drosophila, homolog of antibody
- FLJ40239 antibody
Lane 1 : Marker
Lanes 2-3 : Anti-Pin1 antibody (ab12107) at 1 µg/ml
Lane 2 : pin1 Recombinant 0.1ug
Lane 3 : pin1 Recombinant 0.5ug
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 18.2 kDa
Observed band size: ~34 kDa why is the actual band size different from the predicted?
The observed band size is consistent with the expected size of the Pin1 partial recombinant protein used (amino acids 64 - 163 of the human Pin1 sequence, with GST).
This product has been referenced in:
- Bhaskaran N et al. Fbw7a and Fbw7? collaborate to shuttle cyclin E1 into the nucleolus for multiubiquitylation. Mol Cell Biol 33:85-97 (2013). Human . Read more (PubMed: 23109421) »
- Lv L et al. Inhibition of peptidyl-prolyl cis/trans isomerase Pin1 induces cell cycle arrest and apoptosis in vascular smooth muscle cells. Apoptosis 15:41-54 (2010). WB ; Human . Read more (PubMed: 19904610) »