Anti-Pin1 antibody (ab154577)
- Datasheet
- References
- Protocols
Overview
-
Product nameAnti-Pin1 antibody
See all Pin1 primary antibodies -
DescriptionRabbit polyclonal to Pin1
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, ICC/IFmore details
-
Species reactivityReacts with: Human
Predicted to work with: Cow, Xenopus tropicalis
-
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-163 of Human Pin1 (UniProt ID: Q13526).
-
Positive control
- A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A549 cells
-
General notesKeep as concentrated solution.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
- HeLa nuclear extract lysate (ab14655)
- HepG2 nuclear extract lysate (ab14660)
- HeLa whole cell lysate (ab150035)
- HeLa nuclear extract lysate (ab150036)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- A431 whole cell lysate (ab30132)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
- MOLT4 whole cell lysate (ab7912)
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab154577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 18 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
FunctionEssential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
-
Sequence similaritiesContains 1 PpiC domain.
Contains 1 WW domain. -
DomainThe WW domain is required for the interaction with STIL and KIF20B.
-
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus.
- Information by UniProt
-
Database links
- Entrez Gene: 535470 Cow
- Entrez Gene: 5300 Human
- Omim: 601052 Human
- SwissProt: Q5BIN5 Cow
- SwissProt: Q13526 Human
- Unigene: 465849 Human
-
Alternative names
- DOD antibody
- DODO, Drosophila, homolog of antibody
- FLJ40239 antibody
see all
Images
-
Western blot - Anti-Pin1 antibody (ab154577)All lanes : Anti-Pin1 antibody (ab154577) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 18 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Pin1 antibody (ab154577)Immunofluorescence analysis of paraformaldehyde-fixed A549 cell labeling Pin1 with ab154577 at 1/200 dilution. The lower panel was merged with DNA probe.
References
ab154577 has not yet been referenced specifically in any publications.
