Anti-Pin1 antibody (ab154577)

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Overview

  • Product name

    Anti-Pin1 antibody
    See all Pin1 primary antibodies

  • Description

    Rabbit polyclonal to Pin1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Xenopus tropicalis

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-163 of Human Pin1 (UniProt ID: Q13526).

  • Positive control

    • A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A549 cells

  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab154577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.

  • Sequence similarities

    Contains 1 PpiC domain.
    Contains 1 WW domain.

  • Domain

    The WW domain is required for the interaction with STIL and KIF20B.

  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • DOD antibody
      • DODO, Drosophila, homolog of antibody
      • FLJ40239 antibody
      • FLJ77628 antibody
      • MGC10717 antibody
      • NIMA interacting 1 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
      • Peptidyl prolyl cis/trans isomerase NIMA interacting antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody
      • Peptidyl-prolyl cis-trans isomerase pin1 antibody
      • Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
      • Pin 1 antibody
      • Pin1 antibody
      • PIN1_HUMAN antibody
      • PPIase Pin1 antibody
      • Prolyl isomerase antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
      • Protein NIMA interacting 1 antibody
      • Rotamase Pin1 antibody
      • UBL 5 antibody
      • UBL5 antibody
      see all

    Images

    • Western blot - Anti-Pin1 antibody (ab154577)
      Western blot - Anti-Pin1 antibody (ab154577)
      All lanes : Anti-Pin1 antibody (ab154577) at 1/1000 dilution

      Lane 1 : HepG2 whole cell lysate
      Lane 2 : Raji whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 18 kDa



      12% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-Pin1 antibody (ab154577)
      Immunocytochemistry/ Immunofluorescence - Anti-Pin1 antibody (ab154577)
      Immunofluorescence analysis of paraformaldehyde-fixed A549 cell labeling Pin1 with ab154577 at 1/200 dilution. The lower panel was merged with DNA probe.

    References

    ab154577 has not yet been referenced specifically in any publications.

    Publishing research using ab154577? Please let us know so that we can cite the reference in this datasheet.

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