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Anti-Pin1 antibody (ab154577)

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Western blot - Anti-Pin1 antibody (ab154577)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pin1 antibody (ab154577)

Key features and details

  • Rabbit polyclonal to Pin1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant human Pin1 protein (ab51230)

View more associated products

Overview

  • Product name

    Anti-Pin1 antibody
    See all Pin1 primary antibodies
  • Description

    Rabbit polyclonal to Pin1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-163 of Human Pin1 (UniProt ID: Q13526).

  • Positive control

    • A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A549 cells
  • General notes

    Keep as concentrated solution.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • HepG2 nuclear extract lysate (ab14660)
    • HeLa whole cell lysate (ab150035)
    • HeLa nuclear extract lysate (ab150036)
    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • A431 whole cell lysate (ab30132)
    • A431 whole cell lysate (ab7909)
    • MOLT4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant human Pin1 protein (ab51230)
  • Related Products

    • Recombinant human Pin1 protein (ab51230)

Applications

Our Abpromise guarantee covers the use of ab154577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
  • Sequence similarities

    Contains 1 PpiC domain.
    Contains 1 WW domain.
  • Domain

    The WW domain is required for the interaction with STIL and KIF20B.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q13526 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 535470 Cow
    • Entrez Gene: 5300 Human
    • Omim: 601052 Human
    • SwissProt: Q5BIN5 Cow
    • SwissProt: Q13526 Human
    • Unigene: 465849 Human
    • Alternative names

      • DOD antibody
      • DODO, Drosophila, homolog of antibody
      • FLJ40239 antibody
      • FLJ77628 antibody
      • MGC10717 antibody
      • NIMA interacting 1 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
      • Peptidyl prolyl cis/trans isomerase NIMA interacting antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody
      • Peptidyl-prolyl cis-trans isomerase pin1 antibody
      • Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
      • Pin 1 antibody
      • Pin1 antibody
      • PIN1_HUMAN antibody
      • PPIase Pin1 antibody
      • Prolyl isomerase antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
      • Protein NIMA interacting 1 antibody
      • Rotamase Pin1 antibody
      • UBL 5 antibody
      • UBL5 antibody
      see all

    Images

    • Western blot - Anti-Pin1 antibody (ab154577)
      Western blot - Anti-Pin1 antibody (ab154577)
      All lanes : Anti-Pin1 antibody (ab154577) at 1/1000 dilution

      Lane 1 : HepG2 whole cell lysate
      Lane 2 : Raji whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 18 kDa



      12% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-Pin1 antibody (ab154577)
      Immunocytochemistry/ Immunofluorescence - Anti-Pin1 antibody (ab154577)
      Immunofluorescence analysis of paraformaldehyde-fixed A549 cell labeling Pin1 with ab154577 at 1/200 dilution. The lower panel was merged with DNA probe.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab154577? Please let us know so that we can cite the reference in this datasheet.

    ab154577 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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