Anti-Pin1 antibody (ab154577)
Key features and details
- Rabbit polyclonal to Pin1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Pin1 antibody
See all Pin1 primary antibodies -
Description
Rabbit polyclonal to Pin1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-163 of Human Pin1 (UniProt ID: Q13526).
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Positive control
- A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates; A549 cells
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General notes
Keep as concentrated solution.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa nuclear extract lysate (ab14655)
- HepG2 nuclear extract lysate (ab14660)
- HeLa whole cell lysate (ab150035)
- HeLa nuclear extract lysate (ab150036)
- HeLa whole cell lysate (ab29545)
- Raji whole cell lysate (ab30124)
- A431 whole cell lysate (ab30132)
- A431 whole cell lysate (ab7909)
- MOLT4 whole cell lysate (ab7912)
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab154577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 18 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations. -
Sequence similarities
Contains 1 PpiC domain.
Contains 1 WW domain. -
Domain
The WW domain is required for the interaction with STIL and KIF20B. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 535470 Cow
- Entrez Gene: 5300 Human
- Omim: 601052 Human
- SwissProt: Q5BIN5 Cow
- SwissProt: Q13526 Human
- Unigene: 465849 Human
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Alternative names
- DOD antibody
- DODO, Drosophila, homolog of antibody
- FLJ40239 antibody
see all
Images
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Western blot - Anti-Pin1 antibody (ab154577)All lanes : Anti-Pin1 antibody (ab154577) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 18 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Pin1 antibody (ab154577)Immunofluorescence analysis of paraformaldehyde-fixed A549 cell labeling Pin1 with ab154577 at 1/200 dilution. The lower panel was merged with DNA probe.
Protocols
References (0)
ab154577 has not yet been referenced specifically in any publications.
