Key features and details
- Rabbit polyclonal to Pin1
- Suitable for: IHC-P, IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Pin1 antibody
See all Pin1 primary antibodies
DescriptionRabbit polyclonal to Pin1
Tested applicationsSuitable for: IHC-P, IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Monkey
Synthetic peptide corresponding to Human Pin1 (internal sequence).
Database link: Q13526
- Human testis tissue; HeLa cell lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab191271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/3000. Predicted molecular weight: 18 kDa.|
FunctionEssential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
Sequence similaritiesContains 1 PpiC domain.
Contains 1 WW domain.
DomainThe WW domain is required for the interaction with STIL and KIF20B.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- DOD antibody
- DODO, Drosophila, homolog of antibody
- FLJ40239 antibody
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human testis tissue labeling Pin1 using ab191271 at 5 µg/ml.
All lanes : Anti-Pin1 antibody (ab191271) at 1/700 dilution
All lanes : HeLa cell lysate
All lanes : HRP Goat-anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 18 kDa
Exposure time: 15 seconds
15% SDS Page. 15 seconds exposure time.
ab191271 has been referenced in 1 publication.
- Guo YT et al. Predictive Value of Pin1 in Cervical Low-Grade Squamous Intraepithelial Lesions and Inhibition of Pin1 Exerts Potent Anticancer Activity against Human Cervical Cancer. Aging Dis 11:44-59 (2020). PubMed: 32010480