Product nameAnti-Pin1 antibody
See all Pin1 primary antibodies
DescriptionRabbit polyclonal to Pin1
Tested applicationsSuitable for: WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Synthetic peptide within Human Pin1 aa 113-163. The exact sequence is proprietary. NP_006212.1
Database link: Q13526
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245358 was affinity purified using an epitope specific to Pin1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 18 kDa.|
FunctionEssential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
Sequence similaritiesContains 1 PpiC domain.
Contains 1 WW domain.
DomainThe WW domain is required for the interaction with STIL and KIF20B.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- DOD antibody
- DODO, Drosophila, homolog of antibody
- FLJ40239 antibody
All lanes : Anti-Pin1 antibody (ab245358) at 0.4 µg
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 18 kDa
Exposure time: 3 seconds
ab245358 has not yet been referenced specifically in any publications.