Recombinant Anti-Pin1 antibody [EP1479Y] (ab76309)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1479Y] to Pin1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Pin1 antibody [EP1479Y]
See all Pin1 primary antibodies -
Description
Rabbit monoclonal [EP1479Y] to Pin1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, HEK-293, Mouse brain and Rat brain lysates
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1479Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
For unpurified use at 1/500 |
Notes |
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WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). For unpurified use at 1/500 |
Target
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Function
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations. -
Sequence similarities
Contains 1 PpiC domain.
Contains 1 WW domain. -
Domain
The WW domain is required for the interaction with STIL and KIF20B. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5300 Human
- Entrez Gene: 23988 Mouse
- Entrez Gene: 298696 Rat
- Omim: 601052 Human
- SwissProt: Q13526 Human
- SwissProt: Q9QUR7 Mouse
- Unigene: 465849 Human
- Unigene: 7906 Mouse
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Alternative names
- DOD antibody
- DODO, Drosophila, homolog of antibody
- FLJ40239 antibody
see all
Images
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All lanes : Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : Mouse brain lysate
Lane 4 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa -
Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/500 dilution + HEK-293 cell lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab76309 has been referenced in 7 publications.
- Mishra M et al. Adaptor Protein p66Shc: A Link Between Cytosolic and Mitochondrial Dysfunction in the Development of Diabetic Retinopathy. Antioxid Redox Signal 30:1621-1634 (2019). PubMed: 30105917
- Nechama M et al. The IL-33-PIN1-IRAK-M axis is critical for type 2 immunity in IL-33-induced allergic airway inflammation. Nat Commun 9:1603 (2018). PubMed: 29686383
- Xu T et al. Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis. Neoplasia 19:216-225 (2017). PubMed: 28167297
- Ezratty EJ et al. A Presenilin-2-ARF4 trafficking axis modulates Notch signaling during epidermal differentiation. J Cell Biol 214:89-101 (2016). PubMed: 27354375
- Guito J et al. The cellular peptidyl-prolyl cis/trans isomerase Pin1 regulates reactivation of Kaposi's sarcoma-associated herpesvirus from latency. J Virol 88:547-58 (2014). IP . PubMed: 24173213
- Ma SL et al. Prolyl isomerase Pin1 promotes amyloid precursor protein (APP) turnover by inhibiting glycogen synthase kinase-3ß (GSK3ß) activity: novel mechanism for Pin1 to protect against Alzheimer disease. J Biol Chem 287:6969-73 (2012). IP ; Human . PubMed: 22184106
- Krishnan N et al. The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate the SLBP-histone mRNA complex linking histone mRNA decay with SLBP ubiquitination. Mol Cell Biol 32:4306-22 (2012). PubMed: 22907757