Overview

  • Product name

    Anti-Pin1 antibody [EP1479Y]
    See all Pin1 primary antibodies
  • Description

    Rabbit monoclonal [EP1479Y] to Pin1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human Pin1 aa 50-150 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, HEK-293, Mouse brain and Rat brain lysates
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab76309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).

For unpurified use at 1/500

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
    • Sequence similarities

      Contains 1 PpiC domain.
      Contains 1 WW domain.
    • Domain

      The WW domain is required for the interaction with STIL and KIF20B.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • DOD antibody
      • DODO, Drosophila, homolog of antibody
      • FLJ40239 antibody
      • FLJ77628 antibody
      • MGC10717 antibody
      • NIMA interacting 1 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
      • Peptidyl prolyl cis/trans isomerase NIMA interacting antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody
      • Peptidyl-prolyl cis-trans isomerase pin1 antibody
      • Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
      • Pin 1 antibody
      • Pin1 antibody
      • PIN1_HUMAN antibody
      • PPIase Pin1 antibody
      • Prolyl isomerase antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
      • Protein NIMA interacting 1 antibody
      • Rotamase Pin1 antibody
      • UBL 5 antibody
      • UBL5 antibody
      see all

    Images

    • All lanes : Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates
      Lane 3 : Mouse brain lysates
      Lane 4 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 18 kDa
      Observed band size: 18 kDa

    • Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/500 dilution + HEK-293 cell lysate at 10 µg

      Secondary
      HRP labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 18 kDa
      Observed band size: 18 kDa

    References

    This product has been referenced in:

    • Nechama M  et al. The IL-33-PIN1-IRAK-M axis is critical for type 2 immunity in IL-33-induced allergic airway inflammation. Nat Commun 9:1603 (2018). Read more (PubMed: 29686383) »
    • Xu T  et al. Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis. Neoplasia 19:216-225 (2017). Read more (PubMed: 28167297) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Rat Ovary sections)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: 0.05% Protease treatment for 5 mins @ 37' C
    Permeabilization
    No
    Specification
    Rat Ovary sections
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Dec 04 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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