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Has this antibody (Anti-Pin1 antibody [EP1479Y] (ab76309)) been used for immunofluorescence in cell culture? If so, what dilution is best?
Asked on Apr 21 2015
The Pin1 antibody ab76309 has been tested for IF staining of cell cultures. We found that a dilution range of 1/100-1/250 produced the best result, with minimal background, using a fluorophore-conjugated secondary antibody for detection. But you may want to test a range of dilutions to see what gives the best result with your samples.
Tom RuyleAbcam Scientific Support
Answered on Apr 21 2015