Question (76561) | Anti-Pin1 antibody [EP1479Y] (ab76309)

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Question

Has this antibody (Anti-Pin1 antibody [EP1479Y] (ab76309)) been used for immunofluorescence in cell culture? If so, what dilution is best?

Answer

The Pin1 antibody ab76309 has been tested for IF staining of cell cultures. We found that a dilution range of 1/100-1/250 produced the best result, with minimal background, using a fluorophore-conjugated secondary antibody for detection. But you may want to test a range of dilutions to see what gives the best result with your samples.

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