Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Pin1 antibody [EP1479Y]

Average
Abreviews
This product is known to not work in this application or species.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Rat Ovary sections)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: 0.05% Protease treatment for 5 mins @ 37' C
Permeabilization
No
Specification
Rat Ovary sections
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
Fixative
Formaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1.5% milk
Dilution
1/400

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

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Submitted Dec 04 2015

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