Recombinant Anti-Pin1 antibody [EPR18546-317] (ab192036)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18546-317] to Pin1
- Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Pin1 antibody [EPR18546-317]
See all Pin1 primary antibodies -
Description
Rabbit monoclonal [EPR18546-317] to Pin1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: HeLa, MCF7, C6 and NIH/3T3 cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; mouse and rat brain, heart, kidney and spleen lysates. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt (intra).: HeLa cells. IP: NIH/3T3 cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18546-317 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192036 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
1/2000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
|
ICC/IF |
1/100.
|
|
IP |
1/30.
|
|
Flow Cyt (Intra) |
1/500.
|
Notes |
---|
WB
1/2000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
ICC/IF
1/100. |
IP
1/30. |
Flow Cyt (Intra)
1/500. |
Target
-
Function
Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations. -
Sequence similarities
Contains 1 PpiC domain.
Contains 1 WW domain. -
Domain
The WW domain is required for the interaction with STIL and KIF20B. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5300 Human
- Entrez Gene: 23988 Mouse
- Entrez Gene: 298696 Rat
- Omim: 601052 Human
- SwissProt: Q13526 Human
- SwissProt: Q9QUR7 Mouse
- Unigene: 465849 Human
- Unigene: 7906 Mouse
-
Alternative names
- DOD antibody
- DODO, Drosophila, homolog of antibody
- FLJ40239 antibody
see all
Images
-
Pin1 was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryonic fibroblast cell line) lysate with ab192036 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab192036 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate 10 µg (Input).
Lane 2: ab192036 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab192036 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 2 seconds.
-
All lanes : Anti-Pin1 antibody [EPR18546-317] (ab192036) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
All lanes : Anti-Pin1 antibody [EPR18546-317] (ab192036) at 1/5000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 3 : C6 (rat glial tumor cell line) cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 5 : Mouse brain lysate
Lane 6 : Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-3: 15 seconds; Lanes 4-6: 30 seconds.
-
All lanes : Anti-Pin1 antibody [EPR18546-317] (ab192036) at 1/2000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat kidney lysate
Lane 6 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-3: 5 seconds; Lanes 4-6: 15 seconds.
-
Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pin1 with ab192036 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on HeLa cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
-
Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) cells labeling Pin1 with ab192036 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining on NIH/3T3 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
-
Intracellular flow cytometric analysis of 4% PFA-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Pin1with ab192036 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (3)
ab192036 has been referenced in 3 publications.
- Sun Q et al. Pin1 promotes pancreatic cancer progression and metastasis by activation of NF-?B-IL-18 feedback loop. Cell Prolif 53:e12816 (2020). PubMed: 32347623
- Yuan F et al. Xanthan gum protects temporomandibular chondrocytes from IL-1ß through Pin1/NF-?B signaling pathway. Mol Med Rep 22:1129-1136 (2020). PubMed: 32626995
- Wang J et al. Pin1 inhibition reverses the acquired resistance of human hepatocellular carcinoma cells to Regorafenib via the Gli1/Snail/E-cadherin pathway. Cancer Lett 444:82-93 (2019). PubMed: 30583078