RecombinantRabMAb

Recombinant Anti-PIN4 antibody [EPR10033] (ab155283)

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Western blot - Anti-PIN4 antibody [EPR10033] (ab155283)
  • Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] (ab155283)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
  • Anti-PIN4 antibody [EPR10033] (ab155283)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10033] to PIN4
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PIN4 antibody [EPR10033]
    See all PIN4 primary antibodies

  • Description

    Rabbit monoclonal [EPR10033] to PIN4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PIN4. The exact sequence is proprietary.
    Database link: Q9Y237

  • Positive control

    • 293T, Human skeletal muscle, Human fetal liver, Mouse liver, HepG2 and RAW264.7 cell lysates; Human breast and tonsil tissues. ICC/IF: HepG2 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab155283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/150.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.
      Isoform 2 binds to double-stranded DNA.

    • Tissue specificity

      Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed.

    • Sequence similarities

      Belongs to the ppiC/parvulin rotamase family. PIN4 subfamily.
      Contains 1 PpiC domain.

    • Domain

      The PPIase domain enhances mitochondrial targeting.

    • Post-translational
      modifications

      Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus.
      Isoform 2 is sumoylated by SUMO2 and SUMO3.

    • Cellular localization

      Mitochondrion. Mitochondrion matrix. Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide and Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • EPVH antibody
      • Eukaryotic parvulin homolog antibody
      • hEPVH antibody
      • hPar14 antibody
      • hPar17 antibody
      • MGC138486 antibody
      • OTTHUMP00000023527 antibody
      • OTTHUMP00000217483 antibody
      • OTTHUMP00000217485 antibody
      • Par14 antibody
      • Par17 antibody
      • Parvulin 14 antibody
      • Parvulin-14 antibody
      • Parvulin-17 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 4 antibody
      • Peptidyl prolyl cis/trans isomerase EPVH antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 antibody
      • Peptidyl-prolyl cis-trans isomerase PIN4 antibody
      • Peptidyl-prolyl cis/trans isomerase EPVH antibody
      • PIN4 antibody
      • PIN4_HUMAN antibody
      • PPIase Pin4 antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting, 4 (parvulin) antibody
      • Rotamase Pin4 antibody
      see all

    Images

    • Western blot - Anti-PIN4 antibody [EPR10033] (ab155283)
      Western blot - Anti-PIN4 antibody [EPR10033] (ab155283)
      All lanes : Anti-PIN4 antibody [EPR10033] (ab155283) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : Human skeletal muscle lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : Mouse liver lysate
      Lane 5 : HepG2 lysate
      Lane 6 : RAW264.7 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 13 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] (ab155283)
      Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] (ab155283)

      Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling PIN4 with purified ab155283 at 1/150 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)

      Immunohistochemical analysis of paraffin embedded Human breast tissue, labeling PIN4 with ab155283 at a 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling PIN4 with ab155283 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-PIN4 antibody [EPR10033] (ab155283)
      Anti-PIN4 antibody [EPR10033] (ab155283)

    References (2)

    Publishing research using ab155283? Please let us know so that we can cite the reference in this datasheet.

    ab155283 has been referenced in 2 publications.

    • Saeed U  et al. Parvulin 14 and Parvulin 17 Bind to HBx and cccDNA and Upregulate Hepatitis B Virus Replication from cccDNA to Virion in an HBx-Dependent Manner. J Virol 93:N/A (2019). PubMed: 30567987
    • Frattini V  et al. A metabolic function of FGFR3-TACC3 gene fusions in cancer. Nature 553:222-227 (2018). PubMed: 29323298

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