Recombinant
RabMAb

Recombinant Anti-PIN4 antibody [EPR10033] (ab155283)

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Overview

  • Product name

    Anti-PIN4 antibody [EPR10033]
    See all PIN4 primary antibodies

  • Description

    Rabbit monoclonal [EPR10033] to PIN4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PIN4. The exact sequence is proprietary.
    Database link: Q9Y237

  • Positive control

    • 293T, Human skeletal muscle, Human fetal liver, Mouse liver, HepG2 and RAW264.7 cell lysates; Human breast and tonsil tissues. ICC/IF: HepG2 cells

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
ICC/IF 1/150.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.
      Isoform 2 binds to double-stranded DNA.

    • Tissue specificity

      Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed.

    • Sequence similarities

      Belongs to the ppiC/parvulin rotamase family. PIN4 subfamily.
      Contains 1 PpiC domain.

    • Domain

      The PPIase domain enhances mitochondrial targeting.

    • Post-translational
      modifications

      Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus.
      Isoform 2 is sumoylated by SUMO2 and SUMO3.

    • Cellular localization

      Mitochondrion. Mitochondrion matrix. Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide and Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • EPVH antibody
      • Eukaryotic parvulin homolog antibody
      • hEPVH antibody
      • hPar14 antibody
      • hPar17 antibody
      • MGC138486 antibody
      • OTTHUMP00000023527 antibody
      • OTTHUMP00000217483 antibody
      • OTTHUMP00000217485 antibody
      • Par14 antibody
      • Par17 antibody
      • Parvulin 14 antibody
      • Parvulin-14 antibody
      • Parvulin-17 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 4 antibody
      • Peptidyl prolyl cis/trans isomerase EPVH antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 antibody
      • Peptidyl-prolyl cis-trans isomerase PIN4 antibody
      • Peptidyl-prolyl cis/trans isomerase EPVH antibody
      • PIN4 antibody
      • PIN4_HUMAN antibody
      • PPIase Pin4 antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting, 4 (parvulin) antibody
      • Rotamase Pin4 antibody
      see all

    Images

    • Western blot - Anti-PIN4 antibody [EPR10033] (ab155283)
      Western blot - Anti-PIN4 antibody [EPR10033] (ab155283)
      All lanes : Anti-PIN4 antibody [EPR10033] (ab155283) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : Human skeletal muscle lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : Mouse liver lysate
      Lane 5 : HepG2 lysate
      Lane 6 : RAW264.7 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 13 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] (ab155283)
      Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] (ab155283)

      Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling PIN4 with purified ab155283 at 1/150 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)

      Immunohistochemical analysis of paraffin embedded Human breast tissue, labeling PIN4 with ab155283 at a 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling PIN4 with ab155283 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

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