Recombinant
Anti-PIN4 antibody [EPR10033] (ab155283)
- Datasheet
- References (1)
- Protocols
Overview
-
Product nameAnti-PIN4 antibody [EPR10033]
See all PIN4 primary antibodies -
DescriptionRabbit monoclonal [EPR10033] to PIN4
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IHC-P, ICC, IPmore details
Unsuitable for: Flow Cyt -
Species reactivityReacts with: Mouse, Rat, Human
-
Immunogen
Synthetic peptide within Human PIN4. The exact sequence is proprietary.
Database link: Q9Y237 -
Positive control
- 293T, Human skeletal muscle, Human fetal liver, Mouse liver, HepG2 and RAW264.7 cell lysates; Human breast and tonsil tissues.
-
General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20ºC.
-
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR10033
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 13 kDa. | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/250 - 1/500. | |
| IP | 1/10 - 1/100. |
Application notes
Is unsuitable for Flow Cyt.
Target
-
FunctionIsoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.
Isoform 2 binds to double-stranded DNA. -
Tissue specificityIsoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed.
-
Sequence similaritiesBelongs to the ppiC/parvulin rotamase family. PIN4 subfamily.
Contains 1 PpiC domain. -
DomainThe PPIase domain enhances mitochondrial targeting.
-
Post-translational
modificationsPhosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus.
Isoform 2 is sumoylated by SUMO2 and SUMO3. -
Cellular localizationMitochondrion. Mitochondrion matrix. Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide and Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.
- Information by UniProt
-
Database links
- Entrez Gene: 5303 Human
- Entrez Gene: 628161 Mouse
- Entrez Gene: 69713 Mouse
- Omim: 300252 Human
- SwissProt: Q9Y237 Human
- SwissProt: Q9CWW6 Mouse
- Unigene: 655623 Human
- Unigene: 18298 Mouse
see all -
Alternative names
- EPVH antibody
- Eukaryotic parvulin homolog antibody
- hEPVH antibody
see all
Images
-
![Western blot - Anti-PIN4 antibody [EPR10033] (ab155283)](https://www.abcam.com/ps/products/155/ab155283/Images/PIN4-Primary-antibodies-ab155283-1.jpg)
Western blot - Anti-PIN4 antibody [EPR10033] (ab155283)All lanes : Anti-PIN4 antibody [EPR10033] (ab155283) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Mouse liver lysate
Lane 5 : HepG2 lysate
Lane 6 : RAW264.7 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 13 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)](https://www.abcam.com/ps/products/155/ab155283/Images/PIN4-Primary-antibodies-ab155283-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)Immunohistochemical analysis of paraffin embedded Human breast tissue, labeling PIN4 with ab155283 at a 1/250 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)](https://www.abcam.com/ps/products/155/ab155283/Images/PIN4-Primary-antibodies-ab155283-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling PIN4 with ab155283 at 1/250 dilution.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Frattini V et al. A metabolic function of FGFR3-TACC3 gene fusions in cancer. Nature 553:222-227 (2018). Read more (PubMed: 29323298) »
