Recombinant
RabMAb

Anti-PIN4 antibody [EPR10033] (ab155283)

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Overview

  • Product name
    Anti-PIN4 antibody [EPR10033]
    See all PIN4 primary antibodies
  • Description
    Rabbit monoclonal [EPR10033] to PIN4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide, corresponding to residues in Human PIN4 (UniProt: Q9Y237).

  • Positive control
    • 293T, Human skeletal muscle, Human fetal liver, Mouse liver, HepG2 and RAW264.7 cell lysates; Human breast and tonsil tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function
      Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.
      Isoform 2 binds to double-stranded DNA.
    • Tissue specificity
      Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed.
    • Sequence similarities
      Belongs to the ppiC/parvulin rotamase family. PIN4 subfamily.
      Contains 1 PpiC domain.
    • Domain
      The PPIase domain enhances mitochondrial targeting.
    • Post-translational
      modifications
      Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus.
      Isoform 2 is sumoylated by SUMO2 and SUMO3.
    • Cellular localization
      Mitochondrion. Mitochondrion matrix. Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide and Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • EPVH antibody
      • Eukaryotic parvulin homolog antibody
      • hEPVH antibody
      • hPar14 antibody
      • hPar17 antibody
      • MGC138486 antibody
      • OTTHUMP00000023527 antibody
      • OTTHUMP00000217483 antibody
      • OTTHUMP00000217485 antibody
      • Par14 antibody
      • Par17 antibody
      • Parvulin 14 antibody
      • Parvulin-14 antibody
      • Parvulin-17 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 4 antibody
      • Peptidyl prolyl cis/trans isomerase EPVH antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 antibody
      • Peptidyl-prolyl cis-trans isomerase PIN4 antibody
      • Peptidyl-prolyl cis/trans isomerase EPVH antibody
      • PIN4 antibody
      • PIN4_HUMAN antibody
      • PPIase Pin4 antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting, 4 (parvulin) antibody
      • Rotamase Pin4 antibody
      see all

    Images

    • Western blot - Anti-PIN4 antibody [EPR10033] (ab155283)
      Western blot - Anti-PIN4 antibody [EPR10033] (ab155283)
      All lanes : Anti-PIN4 antibody [EPR10033] (ab155283) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : Human skeletal muscle lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : Mouse liver lysate
      Lane 5 : HepG2 lysate
      Lane 6 : RAW264.7 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 13 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
      Immunohistochemical analysis of paraffin embedded Human breast tissue, labeling PIN4 with ab155283 at a 1/250 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (ab155283)
      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling PIN4 with ab155283 at 1/250 dilution.

    References

    ab155283 has not yet been referenced specifically in any publications.

    Publishing research using ab155283? Please let us know so that we can cite the reference in this datasheet.

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