Recombinant
RabMAb

Recombinant Anti-PIN4 antibody [EPR10033] (Alexa Fluor® 647) (ab225213)

Overview

  • Product name

    Anti-PIN4 antibody [EPR10033] (Alexa Fluor® 647)
    See all PIN4 primary antibodies
  • Description

    Rabbit monoclonal [EPR10033] to PIN4 (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PIN4. The exact sequence is proprietary.
    Database link: Q9Y237

  • Positive control

    • ICC/IF: HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Target

  • Function

    Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.
    Isoform 2 binds to double-stranded DNA.
  • Tissue specificity

    Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed.
  • Sequence similarities

    Belongs to the ppiC/parvulin rotamase family. PIN4 subfamily.
    Contains 1 PpiC domain.
  • Domain

    The PPIase domain enhances mitochondrial targeting.
  • Post-translational
    modifications

    Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus.
    Isoform 2 is sumoylated by SUMO2 and SUMO3.
  • Cellular localization

    Mitochondrion. Mitochondrion matrix. Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide and Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.
  • Information by UniProt
  • Database links

  • Alternative names

    • EPVH antibody
    • Eukaryotic parvulin homolog antibody
    • hEPVH antibody
    • hPar14 antibody
    • hPar17 antibody
    • MGC138486 antibody
    • OTTHUMP00000023527 antibody
    • OTTHUMP00000217483 antibody
    • OTTHUMP00000217485 antibody
    • Par14 antibody
    • Par17 antibody
    • Parvulin 14 antibody
    • Parvulin-14 antibody
    • Parvulin-17 antibody
    • Peptidyl prolyl cis trans isomerase NIMA interacting 4 antibody
    • Peptidyl prolyl cis/trans isomerase EPVH antibody
    • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 antibody
    • Peptidyl-prolyl cis-trans isomerase PIN4 antibody
    • Peptidyl-prolyl cis/trans isomerase EPVH antibody
    • PIN4 antibody
    • PIN4_HUMAN antibody
    • PPIase Pin4 antibody
    • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting, 4 (parvulin) antibody
    • Rotamase Pin4 antibody
    see all

Images

  • ab225213 staining PIN4 in HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab225213 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue)

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab225213 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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