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RecombinantRabMAb

Recombinant Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)

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Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)
  • Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10033] to PIN4 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Protein
Product image
Recombinant human PIN4 protein (ab105618)
Conjugation
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Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-PIN4 antibody [EPR10033] - BSA and Azide free
    See all PIN4 primary antibodies
  • Description

    Rabbit monoclonal [EPR10033] to PIN4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast tissue. ICC/IF: HepG2 cells
  • General notes

    Ab238927 is the carrier-free version of ab155283. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238927 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10033
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Mito. Translation
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial translation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism

Associated products

  • Alternative Versions

    • Anti-PIN4 antibody [EPR10033] (ab155283)
    • Alexa Fluor® 647 Anti-PIN4 antibody [EPR10033] (ab225213)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Mouse liver tissue lysate - total protein (ab29301)
    • RAW 264.7 whole cell lysate (ab7187)
  • Recombinant Protein

    • Recombinant human PIN4 protein (ab105618)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab238927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.
      Isoform 2 binds to double-stranded DNA.
    • Tissue specificity

      Isoform 2 is much more stable than isoform 1 (at protein level). Ubiquitous. Isoform 1 and isoform 2 are expressed in kidney, liver, blood vessel, brain, mammary gland, skeletal muscle, small intestine and submandibularis. Isoform 1 transcripts are much more abundant than isoform 2 in each tissue analyzed.
    • Sequence similarities

      Belongs to the ppiC/parvulin rotamase family. PIN4 subfamily.
      Contains 1 PpiC domain.
    • Domain

      The PPIase domain enhances mitochondrial targeting.
    • Post-translational
      modifications

      Phosphorylated. Isoform 1 phosphorylation occurs both in the nucleus and the cytoplasm. Isoform 1 phosphorylation at Ser-19 does not affect its PPIase activity but is required for nuclear localization, and the dephosphorylation is a prerequisite for the binding to DNA. The unphosphorylated isoform 1 associates with the pre-rRNP complexes in the nucleus.
      Isoform 2 is sumoylated by SUMO2 and SUMO3.
    • Cellular localization

      Mitochondrion. Mitochondrion matrix. Imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein. Directed to mitochondria by a novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide and Nucleus > nucleolus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. Colocalizes in the nucleolus during interphase and on the spindle apparatus during mitosis with NPM1.
    • Target information above from: UniProt accession Q9Y237 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 5303 Human
      • Entrez Gene: 628161 Mouse
      • Entrez Gene: 69713 Mouse
      • Omim: 300252 Human
      • SwissProt: Q9Y237 Human
      • SwissProt: Q9CWW6 Mouse
      • Unigene: 655623 Human
      • Unigene: 18298 Mouse
      • Unigene: 371635 Mouse
      see all
    • Alternative names

      • EPVH antibody
      • Eukaryotic parvulin homolog antibody
      • hEPVH antibody
      • hPar14 antibody
      • hPar17 antibody
      • MGC138486 antibody
      • OTTHUMP00000023527 antibody
      • OTTHUMP00000217483 antibody
      • OTTHUMP00000217485 antibody
      • Par14 antibody
      • Par17 antibody
      • Parvulin 14 antibody
      • Parvulin-14 antibody
      • Parvulin-17 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 4 antibody
      • Peptidyl prolyl cis/trans isomerase EPVH antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 antibody
      • Peptidyl-prolyl cis-trans isomerase PIN4 antibody
      • Peptidyl-prolyl cis/trans isomerase EPVH antibody
      • PIN4 antibody
      • PIN4_HUMAN antibody
      • PPIase Pin4 antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting, 4 (parvulin) antibody
      • Rotamase Pin4 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)
      Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)

      Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling PIN4 with purified ab155283 at 1/150 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155283).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling PIN4 with ab155283 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155283).

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)

      Immunohistochemical analysis of paraffin embedded Human breast tissue, labeling PIN4 with ab155283 at a 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155283).

      Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

    • Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)
      Anti-PIN4 antibody [EPR10033] - BSA and Azide free (ab238927)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab238927? Please let us know so that we can cite the reference in this datasheet.

    ab238927 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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