Recombinant Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1943Y] to PINCH 1 + PINCH 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] -
Description
Rabbit monoclonal [EP1943Y] to PINCH 1 + PINCH 2 -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 73% homology with PINCH 2. Cross-reactivity with this protein has not been confirmed experimentally.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged Human full length recombinant LIMS1 and LIMS2 proteins, HeLa, Jurkat, HEK-293, and Mouse and Rat kidney lysate.
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General notes
This product has switched from a hybridoma to recombinant production method on 7th September 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1943Y -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/5000.
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Notes |
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WB
1/1000 - 1/5000. |
Target
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Cellular localization
PINCH 1: Cell junction > focal adhesion. Cell membrane. PINCH-2: Nucleus. Cell junction > focal adhesion. Cell membrane. -
Database links
- Entrez Gene: 3987 Human
- Entrez Gene: 55679 Human
- Entrez Gene: 110829 Mouse
- Entrez Gene: 225341 Mouse
- Entrez Gene: 361303 Rat
- Entrez Gene: 499443 Rat
- Omim: 602567 Human
- Omim: 607908 Human
see all
Images
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All lanes : Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/5000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole lysate
Lane 2 : Mouse kidney lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole lysate
Lane 3 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/1000 dilution
Lane 1 : His-tagged Human full length recombinant LIMS1 protein, 20 ng
Lane 2 : His-tagged Human full length recombinant LIMS2 protein, 20 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 34 and 37 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab76112 has been referenced in 1 publication.
- Draicchio F et al. Integrin-associated ILK and PINCH1 protein content are reduced in skeletal muscle of maintenance haemodialysis patients. J Physiol 598:5701-5716 (2020). PubMed: 32969494