Overview

  • Product name

    Anti-PINCH 1 antibody [EP1943Y]
    See all PINCH 1 primary antibodies
  • Description

    Rabbit monoclonal [EP1943Y] to PINCH 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human PINCH 1 aa 100-200.

  • Positive control

    • Jurkat, K562, HeLa and 293 cell lysates.
  • General notes

    Previously labelled as LIMS1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab76112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 34-37 kDa (predicted molecular weight: 37 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
    • Tissue specificity

      Expressed in most tissues except in the brain.
    • Sequence similarities

      Contains 5 LIM zinc-binding domains.
    • Cellular localization

      Cell junction > focal adhesion. Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2310016J22Rik antibody
      • 4921524A02Rik antibody
      • AI507642 antibody
      • AU021743 antibody
      • AW551584 antibody
      • C430041B13Rik antibody
      • LIM and senescent cell antigen like domains 1 antibody
      • LIM and senescent cell antigen-like-containing domain protein 1 antibody
      • LIM zinc finger domain containing 1 antibody
      • LIM-type zinc finger domains 1 antibody
      • Lims1 antibody
      • LIMS1_HUMAN antibody
      • Lims1l antibody
      • Particularly interesting new Cys His protein 1 antibody
      • Particularly interesting new Cys His protein antibody
      • Particularly interesting new Cys-His protein 1 antibody
      • PINCH 1 antibody
      • PINCH antibody
      • PINCH-1 antibody
      • PINCH1 antibody
      • Renal carcinoma antigen NY REN 48 antibody
      • Renal carcinoma antigen NY-REN-48 antibody
      • senescent cell antigen antibody
      • wu:fc32c03 antibody
      • zgc:112533 antibody
      see all

    Images

    • All lanes : Anti-PINCH 1 antibody [EP1943Y] (ab76112) at 1/5000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : 293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 37 kDa
      Observed band size: 34-37 kDa
      why is the actual band size different from the predicted?

    References

    ab76112 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab76112.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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