Product nameAnti-PINCH 1 antibody [EP1943Y]
See all PINCH 1 primary antibodies
DescriptionRabbit monoclonal [EP1943Y] to PINCH 1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human PINCH 1 aa 100-200.
- Jurkat, K562, HeLa and 293 cell lysates.
Previously labelled as LIMS1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 34-37 kDa (predicted molecular weight: 37 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionEffector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
Tissue specificityExpressed in most tissues except in the brain.
Sequence similaritiesContains 5 LIM zinc-binding domains.
Cellular localizationCell junction > focal adhesion. Cell membrane.
- Information by UniProt
- 2310016J22Rik antibody
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All lanes : Anti-PINCH 1 antibody [EP1943Y] (ab76112) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 34-37 kDa why is the actual band size different from the predicted?
ab76112 has not yet been referenced specifically in any publications.