Key features and details
- Rabbit monoclonal [EP1944] to PINCH 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-PINCH 1 antibody [EP1944]
See all PINCH 1 primary antibodies
DescriptionRabbit monoclonal [EP1944] to PINCH 1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human PINCH 1 aa 300-400. The exact sequence is proprietary.
- 293T, HeLa, Jurkat, Human fetal kidney, and Human spleen cell lysates. Human kidney tissue. HeLa cells.
Previously labelled as LIMS1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionEffector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
Tissue specificityExpressed in most tissues except in the brain.
Sequence similaritiesContains 5 LIM zinc-binding domains.
Cellular localizationCell junction > focal adhesion. Cell membrane.
- Information by UniProt
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All lanes : Anti-PINCH 1 antibody [EP1944] (ab108609) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human fetal kidney lysate
Lane 5 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab108609 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunofluorescent staining of HeLa cells using ab108609 at 1/100 dilution.
ab108609 has been referenced in 2 publications.
- Mekhdjian AH et al. Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix. Mol Biol Cell 28:1467-1488 (2017). PubMed: 28381423
- Li A et al. The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development. Proteomics 12:203-25 (2012). PubMed: 22253135