Overview

  • Product name

    Anti-PINCH 1 antibody [EP1944]
    See all PINCH 1 primary antibodies
  • Description

    Rabbit monoclonal [EP1944] to PINCH 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human PINCH 1 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • 293T, HeLa, Jurkat, Human fetal kidney, and Human spleen cell lysates. Human kidney tissue. HeLa cells.
  • General notes

    Previously labelled as LIMS1. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab108609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Effector of integrin and growth factor signaling, coupling surface receptors to downstream signaling molecules involved in the regulation of cell survival, cell proliferation and cell differentiation. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway.
    • Tissue specificity

      Expressed in most tissues except in the brain.
    • Sequence similarities

      Contains 5 LIM zinc-binding domains.
    • Cellular localization

      Cell junction > focal adhesion. Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2310016J22Rik antibody
      • 4921524A02Rik antibody
      • AI507642 antibody
      • AU021743 antibody
      • AW551584 antibody
      • C430041B13Rik antibody
      • LIM and senescent cell antigen like domains 1 antibody
      • LIM and senescent cell antigen-like-containing domain protein 1 antibody
      • LIM zinc finger domain containing 1 antibody
      • LIM-type zinc finger domains 1 antibody
      • Lims1 antibody
      • LIMS1_HUMAN antibody
      • Lims1l antibody
      • Particularly interesting new Cys His protein 1 antibody
      • Particularly interesting new Cys His protein antibody
      • Particularly interesting new Cys-His protein 1 antibody
      • PINCH 1 antibody
      • PINCH antibody
      • PINCH-1 antibody
      • PINCH1 antibody
      • Renal carcinoma antigen NY REN 48 antibody
      • Renal carcinoma antigen NY-REN-48 antibody
      • senescent cell antigen antibody
      • wu:fc32c03 antibody
      • zgc:112533 antibody
      see all

    Images

    • All lanes : Anti-PINCH 1 antibody [EP1944] (ab108609) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Human fetal kidney lysate
      Lane 5 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 37 kDa
      Observed band size: 35 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab108609 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunofluorescent staining of HeLa cells using ab108609 at 1/100 dilution.

    References

    This product has been referenced in:

    • Li A  et al. The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development. Proteomics 12:203-25 (2012). Read more (PubMed: 22253135) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab108609.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up