Key features and details
- Mouse monoclonal [N4/15] to PINK1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Product nameAnti-PINK1 antibody [N4/15]
See all PINK1 primary antibodies
DescriptionMouse monoclonal [N4/15] to PINK1
Specificity>30% identity with DMPK.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human PINK1 aa 112-496. NP_115785.1.
EEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQS IGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGA PAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKS KRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHG RTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHR DLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNG CLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHL ESRSYQEAQLPALPESVPPDVRQLVRALLQREASK
Database link: Q9BXM7
- WB: Rat brain lysate. ICC/IF: SK-N-BE cells.
The clone number has been updated from S4-15 to N4/15, both clone numbers name the same antibody clone.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab186303 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 63 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionProtects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitement to dysfunctional mitochondria to initiate their degradation.
Tissue specificityHighly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
Involvement in diseaseDefects in PINK1 are the cause of Parkinson disease type 6 (PARK6) [MIM:605909]. A neurodegenerative disorder characterized by parkinsonian signs such as rigidity, resting tremor and bradykinesia. A subset of patients manifest additional symptoms including hyperreflexia, autonomic instability, dementia and psychiatric disturbances. Symptoms show diurnal fluctuation and can improve after sleep.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Cellular localizationMitochondrion outer membrane. Cytoplasm > cytosol.
- Information by UniProt
- BRPK antibody
- FLJ27236 antibody
- mitochondrial antibody
SK-N-BE (Human neuroblastoma cell line) cells stained for PINK1 using ab186303 at a dilution of 1/100 (1 hour at RT) in ICC/IF.
Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 1 hour at RT.
Anti-PINK1 antibody [N4/15] (ab186303) at 1/200 dilution + Rat brain lysate at 15 µg
Goat Anti-Mouse IgG: HRP at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 63 kDa
Exposure time: 6 minutes
Block: 2% BSA and 2% skimmed milk in 1X TBST.
Incubated with primary antibody for 16 hours at 4°C.
Incubated with secondary antibody for 1 hour at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab186303 has been referenced in 2 publications.
- Balasubramaniam M et al. Interleukin-1ß drives NEDD8 nuclear-to-cytoplasmic translocation, fostering parkin activation via NEDD8 binding to the P-ubiquitin activating site. J Neuroinflammation 16:275 (2019). PubMed: 31882005
- Che Y et al. Role of autophagy in a model of obesity: A long-term high fat diet induces cardiac dysfunction. Mol Med Rep 18:3251-3261 (2018). PubMed: 30066870