Overview

  • Product name

  • Description

    Goat polyclonal to PINX1/LPTS
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human PINX1/LPTS aa 2-15 (N terminal).
    Sequence:

    SMLAERRRKQKWAV-C


    (Peptide available as ab22903)

  • Positive control

    • Recombinant Human PINX1/LPTS protein (ab85338) can be used as a positive control in WB.
  • General notes

    Principal Names – PINX1/LPTS; PIN2-interacting protein 1; LPTL; LPTS; MGC8850; FLJ20565; 67-11-3 protein; hepatocellular carcinoma-related putative tumor suppressor. Official Gene Symbol - PINX1/LPTS (interim symbol). GenBank Accession Number – NP_060354. LocusLink ID - 54984; 72400 (mouse). Please note: We have been contacted by a researcher working with PINX1/LPTS to advise that recent research has shown that there are two splice variants of PINX1/LPTS, one at 45 kDa (full lenth) and the other at 20 kDa (C-terminus truncated). This antibody detects a band at 37kDa in Jurkat Lysate. The researcher is currently testing this antibody to confirm that the band we find at 37 kDa is infact PINX1/LPTS. We would be interested to hear back from any other researchers working with PINX1/LPTS. 11 March, 2004.

     This product was previously labelled as PINX1

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.05% Tris, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).

Approx 35kDa bands seen in HeLa lysate.

Target

  • Function

    Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor.
  • Tissue specificity

    Ubiquitous; expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.
  • Sequence similarities

    Belongs to the PINX1 family.
    Contains 1 G-patch domain.
  • Domain

    The TID (telomerase inhibiting domain) domain is sufficient to bind TERT and inhibit its activity.
    The TBM domain mediates interaction with TERF1.
  • Cellular localization

    Nucleus. Nucleus > nucleolus. Chromosome > telomere. Chromosome > centromere > kinetochore. Localizes in nucleoli, at telomere speckles and to the outer plate of kinetochores. Localization to the kinetochore is mediated by its central region and depends on NDC80 and CENPE.
  • Information by UniProt
  • Database links

  • Alternative names

    • 67-11-3 protein antibody
    • FLJ20565 antibody
    • Hepatocellular carcinoma-related putative tumor suppressor antibody
    • Liver-related putative tumor suppressor antibody
    • LPTL antibody
    • LPTS antibody
    • MGC8850 antibody
    • OTTHUMP00000224984 antibody
    • OTTHUMP00000224985 antibody
    • OTTHUMP00000224986 antibody
    • OTTHUMP00000225053 antibody
    • OTTHUMP00000225054 antibody
    • PIN2 interacting protein 1 antibody
    • Pin2-interacting protein X1 antibody
    • PIN2/TERF1-interacting telomerase inhibitor 1 antibody
    • PINX1 antibody
    • PINX1_HUMAN antibody
    • Protein 67-11-3 antibody
    • TRF1-interacting protein 1 antibody
    see all

Images

  • ab2344 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. 

References

This product has been referenced in:

  • Herrmann G  et al. Conserved interactions of the splicing factor Ntr1/Spp382 with proteins involved in DNA double-strand break repair and telomere metabolism. Nucleic Acids Res 35:2321-32 (2007). Read more (PubMed: 17389648) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

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Thank you for your enquiry. I'm afraid that the only image (and size) that we have have is the one located on the online datasheet. If you have any more questions, please contact us again.

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Please contact our distributor for China with regards to your order requirements:- Jingmei Biotech 14Fl., Kaiyuan Bldg No.7001 Beihuan Da Dao Shenzhen 518034 CHINA Phone: 086755-8354 6191/5/89 Toll Free: 800-830-5060 Fax: 086755-8354 6196 Email: shenzhen@jingmei.com Web: www.jingmei.com Beijing Phone: 08610-6552 0121 Fax: 6552 5169 Email: Beijing@jingmei.com Shanghai Phone: 08621-6466 4434, 6415 8521/43 Fax: 6415 8543 Email: shanghai@jingmei.com Guangzhou Phone: 08620-8134 0034 Fax: 8134 0624 Email: Guangzhou@jingmei.com

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This antibody has not been tested in IHC. This means that we have not tested it but it does not mean that it will not work in this application. We have no further information on this. If you still wish to purchase the antibody there is a detailed ordering section on the website which will explain how to order and answer any questions you have.

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in immunohistochemistry . All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any more questions, please contact us again.

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