Western blot abreview for Anti-PIP2 antibody [2C11]

Inconclusive
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human lung epithelial cell)
Loading amount
50 µg
Specification
Human lung epithelial cell
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/200
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST with 5% skim milk

Secondary antibody

Name
Non-Abcam antibody was used: Anti mouse antibody
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Exposure
15 minute(s) and 0 second(s)

Additional data

Additional Notes
I bought this antibody for western blot several days ago. but i couldn't detected PIP2. The problem is that there was no mention about PIP2 size in information sheet. when i searched, I found that PIP2 is just 1098.19 (about 1KDa). But your answer in inquire is about 105KDa. There is big difference, isn't it? Also, some paper showed that PIP2 western blot size is 27KDa (used Ab from different company).

So, I was really confused.

I would appreciate it if you give correct answer about band size(MW) detected by this Ab and any special protocol for PIP2 western blot.
Abcam response
Thank you for submitting your Abreview. We sincerely apologize for the incorrect information on our datasheet that was misleading you.
This antibody is not suitable in standard Western blotting, since it was raised against a lipid (not a protein or peptide) which is too small - as you correctly noticed - to be detectable in a Western blot. Instead a lipid dot assay should be used with this antibody (see references: PMID 10917659 and 11559758).
Our answer to the enquiry on the datasheet (Enquiries-Tab) has now been corrected, and WB as tested application has been removed.
We are happy to offer you a replacement or refund for this antibody, and apologize for the inconvenience this has caused.

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Verified customer

Submitted Jan 14 2009

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