Immunocytochemistry/ Immunofluorescence abreview for Anti-PIP2 antibody [2C11]

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (organ of Corti)
Specification
organ of Corti
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C

Other product details

Dilution
1/50
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/1%BSA

Secondary antibody

Name
Non-Abcam antibody was used: Alexa fluor 488 conjugated goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400

Additional data

Additional Notes
Figure:
PiP2 staining in the hair bundle (stained with phaloidin (red)) of an inner ear sensory hair cell. Scale bar : 5 µm.

Dr. Vincent Michel

Verified customer

Submitted Dec 01 2010

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